Chlamydomonas reinhardtii heat stress time course experiment
Experimental design
Figure 1: Experimental design of the heat treatment experiments (Zhang et al.)
Publication
Title: Systems-wide analysis revealed shared and unique responses to moderate and acute high temperatures in the green alga Chlamydomonas reinhardtii.
Authors: Zhang N, Mattoon EM, McHargue W, Venn B, Zimmer D, Pecani K, Jeong J, Anderson CM, Chen C, Berry JC, Xia M, Tzeng SC, Becker E, Pazouki L, Evans B, Cross F, Cheng J, Czymmek KJ, Schroda M, Mühlhaus T, Zhang R.
Journal: Commun Biol. 2022 May 13;5(1):460. doi: 10.1038/s42003-022-03359-z. PMID: 35562408; PMCID: PMC9106746.
Sampling schema
| time point [h] | condition | temperature [°C] | sample name | description |
|---|---|---|---|---|
| -20 | control | 25 | pre1 | |
| -18 | control | 25 | pre2 | |
| 0 | start heat treatment | 25 -> 35/40 | HS_T00h | reach treatment temperature |
| 0.5 | heat treatment | 35/40 | HS_T00.5h | - |
| 1 | heat treatment | 35/40 | HS_T01h | - |
| 2 | heat treatment | 35/40 | HS_T02h | - |
| 4 | heat treatment | 35/40 | HS_T04h | - |
| 8 | heat treatment | 35/40 | HS_T08h | - |
| 16 | heat treatment | 35/40 | HS_T16h | - |
| 24 | end treatment | 35/40 -> 25 | HS_T24h | end heat treatment |
| 24 | start recovery | 25 | RE_T00h | RCT (reach control temperature) |
| 26 | recovery | 25 | RE_T02h | - |
| 28 | recovery | 25 | RE_T04h | - |
| 32 | recovery | 25 | RE_T08h | - |
| 48 | recovery | 25 | RE_T24h | - |
| 72 | recovery | 25 | RE_T48h | - |