diff --git a/assays/RelativeWaterContent/README.md b/assays/RelativeWaterContent/README.md
index 3ff0e03c4e73200ee1b6cb7e025ef65c95aa0aae..6c4ce0b1181aa324303af87ac2b34dac7fd9e172 100644
--- a/assays/RelativeWaterContent/README.md
+++ b/assays/RelativeWaterContent/README.md
@@ -1 +1,23 @@
-Leaf relative water content
\ No newline at end of file
+#### Leaf relative water content
+
+
+- An approximately 1 cm 3 section of leaf was sampled from each plot
+and transferred to a pre-weighed Eppendorf tube. 
+
+- The tubes were then reweighed, filled with MilliQ water
+and left at 4 °C overnight. 
+
+- The following morning, the turgid leaf section was removed from the tube, carefully dried with a paper towel and reweighed. 
+
+- Samples were then placed back into the emptied
+Eppendorf tubes and placed in an oven at approximately 70 °C overnight. 
+
+- Once leaf sections were completely
+dry, the tubes containing the leaf samples were reweighed. 
+
+
+- The empty tube weight was subtracted from the
+fresh weight and dried weight measurements and relative water content (RWC, %) was calculated using the
+equation:
+
+$$RWC = \frac{fresh weight - dry weight}{turgid weight - dry weight} ~x ~100 $$ 
\ No newline at end of file