From 049c09423d635c9d9d4763a553a88ae216851c20 Mon Sep 17 00:00:00 2001
From: Kathryn Dumschott <k.dumschott@fz-juelich.de>
Date: Mon, 26 May 2025 09:57:51 +0000
Subject: [PATCH] Edit README.md

---
 assays/RelativeWaterContent/README.md | 24 +++++++++++++++++++++++-
 1 file changed, 23 insertions(+), 1 deletion(-)

diff --git a/assays/RelativeWaterContent/README.md b/assays/RelativeWaterContent/README.md
index 3ff0e03..6c4ce0b 100644
--- a/assays/RelativeWaterContent/README.md
+++ b/assays/RelativeWaterContent/README.md
@@ -1 +1,23 @@
-Leaf relative water content
\ No newline at end of file
+#### Leaf relative water content
+
+
+- An approximately 1 cm 3 section of leaf was sampled from each plot
+and transferred to a pre-weighed Eppendorf tube. 
+
+- The tubes were then reweighed, filled with MilliQ water
+and left at 4 °C overnight. 
+
+- The following morning, the turgid leaf section was removed from the tube, carefully dried with a paper towel and reweighed. 
+
+- Samples were then placed back into the emptied
+Eppendorf tubes and placed in an oven at approximately 70 °C overnight. 
+
+- Once leaf sections were completely
+dry, the tubes containing the leaf samples were reweighed. 
+
+
+- The empty tube weight was subtracted from the
+fresh weight and dried weight measurements and relative water content (RWC, %) was calculated using the
+equation:
+
+$$RWC = \frac{fresh weight - dry weight}{turgid weight - dry weight} ~x ~100 $$ 
\ No newline at end of file
-- 
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