From 31deb5eaac92923f3fb1f1ff8e3425df0d54882a Mon Sep 17 00:00:00 2001
From: Kathryn Dumschott <k.dumschott@fz-juelich.de>
Date: Tue, 10 Jun 2025 11:55:46 +0000
Subject: [PATCH] Update file README.md

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+# Experimental Setup
+
+**Field Trial Location:** 
+- The trial took place at the INIA-Huasco experimental center located in the southern Atacama Desert (Vallenar, Chile, 28°34′ S, 70°47′ W and 469 m.a.s.l.) during the 2019/2020 growing season (sowing on 26 September 2019, harvest on 21 February 2020).
+- The Atacama desert is one of the driest regions in the world, ensuring that rainfall would not influence the irritation treatment of the experiment.
+
+**Soil Characteristics:** 
+- Sandy loam textural class, composed of sand (75.5%), silt (10.9%) and clay (13.7%), with a low organic matter content (2.0%), an alkaline pH (8.1), slight salinity (2.4 dS m−1), and with a basic content of N-P-K (45-21-311 mg kg−1 respectively)
+
+**Experimental Design:**
+- Nine coastal lowland self-pollinated quinoa lines (CLS-1 to CLS-9) and one commercial cultivar (cv Regalona)
+- Plots here 2 x 4 m and contained four rows of quinoa with an inter-row spacing of 0.5 m
+- Three plots of each line were sown for each treatment.
+- Seeds were sown at a density of 15 kg ha−1 with >85% germination.
+- Two treatments: 
+    - <ins>Full Irrigation (FI):</ins> Considered the reposition of ET<sub>0</sub> and the lack of a crop coefficient (K<sub>c</sub>) for quinoa growing in the region.
+    - <ins>Reduced Irrigation (RI):</ins> Defined a severe reduction of irrigation time (50%) and kept the same schedule as for the full irrigation treatment (every second or third day) by means of pressurized drip lines. RI treatment started at growth stage 20 (branching, extended BBCH).
+- Fertilizer was provided at sowing (75-120-60 N-P-K) and an additional 75 N was provided at growth stage 12 (second pair of leaves visible, extended BBCH).
+- Chlorothalonil (2 L ha−1 BRAVO 720, Syngenta) was applied from growth stage 16 (six pairs of leaves visible, extended BBCH) to manage disease.
+
+
+## Figure: Field trial plot layout
+
+![Quinoaplots](quinoa_trialplots.png)
+
+## Data Collected & Corresponding Assays
+
+#### Data recorded throughout the trial included:
+
+- **Weather data:** Collected via meteorlogical station located at the INIA-Huasco experimental setup, approximately 200 meters from the trial.
+    -  See Assay: [Weather_Data](https://git.nfdi4plants.org/usadellab/quinoa_chileanfieldtrial/-/tree/main/assays/Weather_Data?ref_type=heads)    
+
+- **Soil sensors:** Recorded volumetric water content and temperature (located at the yellow stars in the figure above).
+     -  See Assay: [Weather_Data](https://git.nfdi4plants.org/usadellab/quinoa_chileanfieldtrial/-/tree/main/assays/Weather_Data?ref_type=heads)
+
+- **Phenology:** Determined visually via regular inspections of the trial using the extended BBCH scale by Sosa-Zúñiga et al. 2017 [1].
+    - See Assay: [Phenology](https://git.nfdi4plants.org/usadellab/quinoa_chileanfieldtrial/-/tree/main/assays/Phenology?ref_type=heads) 
+
+#### Data Collected at the visible inflorescence stage of development (BBCH 59); 46-50 days after sowing:
+
+- **Morphological traits:** Collected for all plots of both treatments on day 47 and included measurements such as plant height and stem diameter.
+
+     -  See Assay:
+ - **Leaf relative water content:** Collected for all plots of both treatments on day 47.
+    -  See Assay: [RelativeWaterContent](https://git.nfdi4plants.org/usadellab/quinoa_chileanfieldtrial/-/tree/main/assays/RelativeWaterContent?ref_type=heads)    
+
+
+
+## References
+
+[1] Sosa-Zuniga, V.; Brito, V.; Fuentes, F.; Steinfort, U. Phenological growth stages of quinoa (Chenopodium quinoa) based on the BBCH scale. Ann. Appl. Biol. 2017, 171, 117–124. 
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