From f85c849c1bd6485b676d70df3f39a9dd3f38936f Mon Sep 17 00:00:00 2001 From: Kathryn Dumschott <k.dumschott@fz-juelich.de> Date: Mon, 26 May 2025 08:41:03 +0000 Subject: [PATCH] Update file README.md --- assays/MiniPam/README.md | 13 +++++++++++++ 1 file changed, 13 insertions(+) diff --git a/assays/MiniPam/README.md b/assays/MiniPam/README.md index e69de29..d1f7292 100644 --- a/assays/MiniPam/README.md +++ b/assays/MiniPam/README.md @@ -0,0 +1,13 @@ +Fluorescence data was collected using a MINI-PAM-II fluorometer (Walz Heinz GmbH, Effeltrich, +Germany). To conduct measurements, the leaf clip was attached to a leaf and measurements of +photosynthetically active radiation (PAR) and the photosynthetic efficiency of photosystem II (PhiPSII) were +recorded. This was repeated ~10 times per plot. Each FI plot was followed by its RI plot counterpart. Plots of +blocks 1 and 4 were measured 49 days after sowing between 14:45 and 15:45. Plots of blocks 2 and 5 were +measured 48 days after sowing between 15:00 and 17:00. All blocks were measured 50 days after sowing +between 10:24 and 12:49. The fluorescence kinetics of saturation pulse analyses for measurements wereinspected using WinControl-3 Software (version 3.29-rev.1112) (Walz Heinz GmbH, Effeltrich, Germany). +Measurements that did not pass inspection were discarded from subsequent analyses. +PhiPSII values were clustered according to genotype, treatment and PAR level using custom R scripts. +PAR was divided into four levels: low (<500 μmol photons m -2 s -1 ), mid-low (500-1000 μmol photons m -2 s -1 ), +mid-high (1000-1500 μmol photons m -2 s -1 ) and high (>1500 μmol photons m -2 s -1 ). Agglomerative hierarchical +clustering was applied on the scaled PhiPSII means for all plots using the ‘agnes’ function of the R package +‘cluster’ [100] with a cut-off of five clusters. \ No newline at end of file -- GitLab