From 50ed4f9de554ef810704c24f2a20f67cb8d531fb Mon Sep 17 00:00:00 2001 From: Angela Kranz <a.kranz@fz-juelich.de> Date: Sun, 17 Nov 2024 16:27:04 +0000 Subject: [PATCH] Update README.md --- assays/Ribes_nigrum_genome_sequencing/README.md | 7 ++++++- 1 file changed, 6 insertions(+), 1 deletion(-) diff --git a/assays/Ribes_nigrum_genome_sequencing/README.md b/assays/Ribes_nigrum_genome_sequencing/README.md index d837a76..cf5daca 100644 --- a/assays/Ribes_nigrum_genome_sequencing/README.md +++ b/assays/Ribes_nigrum_genome_sequencing/README.md @@ -1,4 +1,9 @@ # Genome sequencing For generating a *Ribes nigrum* genome assembly, the cultivar Rosenthals Langtraubige was used. DNA was extracted and filtered for long fragments above 40 kbp in order to perform Oxford Nanopore (ONT) sequencing and PacBio sequencing. -Briefly, DNA was extracted, short fragments filtered out, DNA purity confirmed, and the libraries were prepared using the standard ONT protocol for the SQK-LSK112 kit. After DNA fragment repair and 3'-adenylation sequencing adapters were ligated to the DNA fragments, before a last purification step with AMPure XP beads. ONT sequencing was performed in-house, PacBio sequencing was performed by Genohub (Brigham Young University, Provo, UT, USA). \ No newline at end of file +Briefly, DNA was extracted, short fragments filtered out, DNA purity confirmed, and the libraries were prepared using the standard ONT protocol for the SQK-LSK112 kit. After DNA fragment repair and 3'-adenylation sequencing adapters were ligated to the DNA fragments, before a last purification step with AMPure XP beads. ONT sequencing was performed in-house, PacBio sequencing was performed by Genohub (Brigham Young University, Provo, UT, USA). + +## Experimental workflow + + + -- GitLab