# *Ribes nigrum* genome sequencing experiment

To generate a blackcurrant genome assembly, DNA of the cultivar *R. nigrum* Rosenthals Langtraubige was used. DNA was extracted from young leaves of a single adult plant. Sequencing was performed with Oxford Nanopore Technologies, PacBio HiFi and Illumina.


## Study metadata

This is an overview about the metadata for the *R. nigrum* genome sequencing experiment. The metadata can also be found in the [isa.study file](https://git.nfdi4plants.org/usadellab/ribes_nigrum_genome/-/blob/main/studies/Ribes_nigrum_genome/isa.study.xlsx?ref_type=heads).

| Input [Source Name] | Characteristic [organism] | Characteristic [plant structure development stage] | Characteristic [plant structure] | Characteristic [geographic area] | Output [Sample Name]
| ------ | ------ | ------ | ------ |------  | ------ 
| R. nigrum cv Rosenthals Langtraubige       | Ribes nigrum       | adult      | leaves | Germany | R. nigrum cv Rosenthals Langtraubige_Leaves


## Experimental set-up

![](./resources/RibesNigrum_Greenhouse.jpeg)


## Related repositories and files

After harvesting of leaves, the following steps were performed:

[DNA isolation](https://git.nfdi4plants.org/usadellab/ribes_nigrum_genome/-/tree/main/assays/Ribes_nigrum_genome_sequencing?ref_type=heads)
[Library preparation](https://git.nfdi4plants.org/usadellab/ribes_nigrum_genome/-/tree/main/assays/Ribes_nigrum_genome_sequencing?ref_type=heads)
[Sequencing](https://git.nfdi4plants.org/usadellab/ribes_nigrum_genome/-/tree/main/assays/Ribes_nigrum_genome_sequencing?ref_type=heads)
[Genome assembly](https://git.nfdi4plants.org/usadellab/ribes_nigrum_genome/-/tree/main/runs/Ribes_nigrum_genome?ref_type=heads)
Genome analysis