From f54232f4763b927525cdba1cc25fc73dfd983494 Mon Sep 17 00:00:00 2001
From: Kathryn Dumschott <k.dumschott@fz-juelich.de>
Date: Thu, 31 Aug 2023 12:14:23 +0000
Subject: [PATCH] Update ExperimentalSetup.md

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 studies/ExperimentalSetup.md | 30 +++++++++++++++++++++++++++++-
 1 file changed, 29 insertions(+), 1 deletion(-)

diff --git a/studies/ExperimentalSetup.md b/studies/ExperimentalSetup.md
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-### Experimental Setup
+# Experimental Setup
+
+**Plant Material**
+
+Seeds of S. lycopersicoides LA2951 and the corresponding IL line seeds were obtained from the Tomato Genetics Resource Center (TGRC; https://tgrc.ucdavis.edu/). 
+
+**Drought Stress Experiment Preparation**
+
+40 plants were grown in groups of eight with randomized positions in a phyto chamber.
+
+Settings:
+
+- Artificial light: 350 μmol m−2 sec−1 for 16 h per day
+- Humidity: maintained at approximately 70% 
+- Temperature: maintained at 22°C (day) and 18°C (night)
+
+Approximately 8-week-old plants were transplanted into approximately 2-L pots and pruned apically at approximately 1 cm above the 11th node. At 10 days after transplanting and pruning, any emergent sucker branch >1 cm in length was removed and plants were recovered for a further 14 days before the start of the experiment. 
+
+**Drought Stress Experiment**
+
+Third and fourth leaf tissues from the first and second apical branches of eight plants were harvested at 11–12 h after dawn (Timepoint in the diurnal cycle [ZT] 11–12) as a time point 0 control (day 0). Pots were all saturated with water at this time by soaking for 5 min and for the next 5 days the weight of control group pots was kept constant by adding measured volumes of water every 1–2 days while drought stress pots were not watered at all. 
+
+The same tissues were then harvested at ZT 11–12 for time point 1 (day 5) from eight drought-stressed and eight control group plants. The remaining eight control plants were again soaked in water for 5 min and for the next 4 days their weight was again kept constant with measured volumes of water, while eight treatment plants were now supplied with 50 ml water each day. 
+
+Tissues were then harvested at ZT 11–12 for time point 2 (day 9). 
+
+Tissue was always pooled for two plants, hence four pools of two plants each representing eight different plants were analyzed. 
+
+Tissues from all time points were first ground with liquid N2 and stored at −80°C until further use. 
-- 
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