
Total RNA was isolated from whole seedlings using the NucleoSpin RNA Plant Kit (Macherey-Nagel). First-strand cDNA was synthesized using the PrimeScript RT Reagent Kit (Perfect Real Time, Takara Bio). Reverse transcription-mediated quantitative real-time PCR (RT–qPCR) analyses were performed on an AriaMx Real-Time PCR System (Agilent) using Absolute Blue Low Rox Mix (Thermo Fisher Scientific). PROTEIN 19 PHOSPHATASE2a subunit A3 (PP2A, AT1G13320) and TIP41 (AT4G34270) were used as reference genes to calculate relative
expression values (2ΔCt values). Primer sequences have been described previously (Anwer et al., 2020). The primers used for PRR7 were forward: 5ʹ-TGAAAGTTGGAAAAGGACCA-3ʹ and reverse:5ʹ-GTTCCACGTGCATTAGCTCT-3ʹ.