diff --git a/.gitattributes b/.gitattributes
index 89a07c76d5f8f22ec21ad4e9be6fcc2d2a276ce0..2635f9cbbd98377405c62ee003f1d6b4b772e7b2 100644
--- a/.gitattributes
+++ b/.gitattributes
@@ -1,34 +1 @@
 **/dataset/** filter=lfs diff=lfs merge=lfs -text
-/assays/Proteomics/dataset/20191113_Zhang_40C_B_sample65.raw filter=lfs diff=lfs merge=lfs -text
-/assays/Proteomics/dataset/20191113_Zhang_40C_B_sample66.raw filter=lfs diff=lfs merge=lfs -text
-/assays/Proteomics/dataset/20191113_Zhang_40C_B_sample67.raw filter=lfs diff=lfs merge=lfs -text
-/assays/Proteomics/dataset/20191113_Zhang_40C_B_sample68.raw filter=lfs diff=lfs merge=lfs -text
-/assays/Proteomics/dataset/20191114_Zhang_40C_B_sample69.raw filter=lfs diff=lfs merge=lfs -text
-/assays/Proteomics/dataset/20191114_Zhang_40C_B_sample70.raw filter=lfs diff=lfs merge=lfs -text
-/assays/Proteomics/dataset/20191114_Zhang_40C_B_sample71.raw filter=lfs diff=lfs merge=lfs -text
-/assays/Proteomics/dataset/20191114_Zhang_40C_B_sample72.raw filter=lfs diff=lfs merge=lfs -text
-/assays/Proteomics/dataset/20191114_Zhang_40C_B_sample73.raw filter=lfs diff=lfs merge=lfs -text
-/assays/Proteomics/dataset/20191114_Zhang_40C_B_sample74.raw filter=lfs diff=lfs merge=lfs -text
-/assays/Proteomics/dataset/20191114_Zhang_40C_B_sample75.raw filter=lfs diff=lfs merge=lfs -text
-/assays/Proteomics/dataset/20191115_Zhang_40C_B_sample76.raw filter=lfs diff=lfs merge=lfs -text
-/assays/Proteomics/dataset/20191115_Zhang_40C_B_sample77.raw filter=lfs diff=lfs merge=lfs -text
-/assays/Proteomics/dataset/20191115_Zhang_40C_B_sample78.raw filter=lfs diff=lfs merge=lfs -text
-/assays/Proteomics/dataset/20191115_Zhang_40C_B_sample79.raw filter=lfs diff=lfs merge=lfs -text
-/assays/Proteomics/dataset/20191115_Zhang_40C_B_sample80.raw filter=lfs diff=lfs merge=lfs -text
-/assays/Proteomics/dataset/20191115_Zhang_40C_C_sample81.raw filter=lfs diff=lfs merge=lfs -text
-/assays/Proteomics/dataset/20191116_Zhang_40C_C_sample82.raw filter=lfs diff=lfs merge=lfs -text
-/assays/Proteomics/dataset/20191116_Zhang_40C_C_sample83.raw filter=lfs diff=lfs merge=lfs -text
-/assays/Proteomics/dataset/20191116_Zhang_40C_C_sample84.raw filter=lfs diff=lfs merge=lfs -text
-/assays/Proteomics/dataset/20191116_Zhang_40C_C_sample85.raw filter=lfs diff=lfs merge=lfs -text
-/assays/Proteomics/dataset/20191116_Zhang_40C_C_sample86.raw filter=lfs diff=lfs merge=lfs -text
-/assays/Proteomics/dataset/20191116_Zhang_40C_C_sample87.raw filter=lfs diff=lfs merge=lfs -text
-/assays/Proteomics/dataset/20191116_Zhang_40C_C_sample88.raw filter=lfs diff=lfs merge=lfs -text
-/assays/Proteomics/dataset/20191117_Zhang_40C_C_sample89.raw filter=lfs diff=lfs merge=lfs -text
-/assays/Proteomics/dataset/20191117_Zhang_40C_C_sample90.raw filter=lfs diff=lfs merge=lfs -text
-/assays/Proteomics/dataset/20191117_Zhang_40C_C_sample91.raw filter=lfs diff=lfs merge=lfs -text
-/assays/Proteomics/dataset/20191117_Zhang_40C_C_sample92.raw filter=lfs diff=lfs merge=lfs -text
-/assays/Proteomics/dataset/20191117_Zhang_40C_C_sample93.raw filter=lfs diff=lfs merge=lfs -text
-/assays/Proteomics/dataset/20191117_Zhang_40C_C_sample94.raw filter=lfs diff=lfs merge=lfs -text
-/assays/Proteomics/dataset/20191118_Zhang_40C_C_sample95.raw filter=lfs diff=lfs merge=lfs -text
-/assays/Proteomics/dataset/20191118_Zhang_40C_C_sample96.raw filter=lfs diff=lfs merge=lfs -text
-/assays/Proteomics/dataset/msmsScans.txt filter=lfs diff=lfs merge=lfs -text
diff --git a/assays/Proteomics/isa.assay.xlsx b/assays/Proteomics/isa.assay.xlsx
index 46612cee4997b4ab12faba5956130eb2a913ecef..50f81b134a374c42c99ea95cecd6c92841d177b4 100644
Binary files a/assays/Proteomics/isa.assay.xlsx and b/assays/Proteomics/isa.assay.xlsx differ
diff --git a/assays/Proteomics/protocols/GradientProtocol.txt b/assays/Proteomics/protocols/GradientProtocol.txt
new file mode 100644
index 0000000000000000000000000000000000000000..056534f1f7411588bb5bf46998dc4b8a249051af
--- /dev/null
+++ b/assays/Proteomics/protocols/GradientProtocol.txt
@@ -0,0 +1,9 @@
+Time = 0–4 min, 2% B isocratic; 
+4–8 min, 2–10% B; 
+8–83 min, 10–25% B;
+83–97 min, 25–50% B; 
+97–105 min, 50–98%
+
+
+Mobile phase consisted of A, 0.1%formic acid; 
+mobile phase B, 0.1% formic acid in acetonitrile. 
\ No newline at end of file
diff --git a/studies/HeatstressExperiment/isa.study.xlsx b/studies/HeatstressExperiment/isa.study.xlsx
index 780510f9480aa534d35a2759846c887d3c3bd660..bd7714d510d2facf28e3ffc98c3e5d023eecb573 100644
Binary files a/studies/HeatstressExperiment/isa.study.xlsx and b/studies/HeatstressExperiment/isa.study.xlsx differ