Chlamydomonas reinhardtii heat stress time course experiment
Experimental design
Figure 1: Experimental design of the heat treatment experiments (Zhang et al. 2022).
Publication
Title
Systems-wide analysis revealed shared and unique responses to moderate and acute high temperatures in the green alga Chlamydomonas reinhardtii.
Citation
Zhang N, Mattoon EM, McHargue W, Venn B, Zimmer D, Pecani K, Jeong J, Anderson CM, Chen C, Berry JC, Xia M, Tzeng SC, Becker E, Pazouki L, Evans B, Cross F, Cheng J, Czymmek KJ, Schroda M, Mühlhaus T, Zhang R. Systems-wide analysis revealed shared and unique responses to moderate and acute high temperatures in the green alga Chlamydomonas reinhardtii. Commun Biol. 2022 May 13;5(1):460. doi: 10.1038/s42003-022-03359-z . PMID: 35562408 ; PMCID: PMC9106746.
Sampling schema
| time point [h] | condition | temperature [°C] | sample name | description | sample taken Proteomics =16 | sample taken Transcriptomics =15 |
|---|---|---|---|---|---|---|
| -20 | control | 25 | pre1 | x | ||
| -18 | control | 25 | pre2 | preheat | x | x |
| 0 | start heat treatment | 25 -> 35/40 | HS_T00h | RHT (reach high temperature) | x | x |
| 0.5 | heat treatment | 35/40 | HS_T00.5h | - | x | x |
| 1 | heat treatment | 35/40 | HS_T01h | - | x | x |
| 2 | heat treatment | 35/40 | HS_T02h | - | x | x |
| 4 | heat treatment | 35/40 | HS_T04h | - | x | x |
| 8 | heat treatment | 35/40 | HS_T08h | - | x | x |
| 16 | heat treatment | 35/40 | HS_T16h | - | x | x |
| 24 | end treatment | 35/40 -> 25 | HS_T24h | end heat treatment | x | x |
| 24 | start recovery | 25 | RE_T00h | RCT (reach control temperature) | x | x |
| 26 | recovery | 25 | RE_T02h | - | x | x |
| 28 | recovery | 25 | RE_T04h | - | x | x |
| 32 | recovery | 25 | RE_T08h | - | x | x |
| 48 | recovery | 25 | RE_T24h | - | x | x |
| 72 | recovery | 25 | RE_T48h | - | x | x |
| Technology | temperature treatments | timepoints | replicates | sample count |
|---|---|---|---|---|
| Proteomics | 2 | 16 | 3 | 96 |
| Transcriptomics | 2 | 15 | 3 | 90 |