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ARC mininmal Example RNASeq

This is a minimal Example ARC packaging an mRNA-Seq dataset with metadata and computations.

Data origin

Additional payload

The following folders are not part of the ARC
for details, see: ARC specs:Additional paylod

Directory  Purpose
_GEO_submission Example metadata files as required for submission to GEO

Notes and ToDos

Experimental metadata in isa.assay.xlsx

  • split GEO SWATE templates into four sheets
    • 1SPL01_plants
    • 2EXT01_RNA
    • 3ASY01_RNASeq
    • 4COM01_RNASeq

Adding large raw data via git lfs

  1. Before addind the files to the ARC, track them via git lfs

    git lfs track "*.fastq.gz"
    git lfs track "01_kallisto_index"    
  2. Move / add the large data files to the respective folders

  3. Add them via git add

    git add assays/Talinum_RNASeq_minimal/dataset/
    git add runs/run1/01_kallisto_index
    
  4. Commit