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Reversible Burst of Transcriptional Changes during Induction of Crassulacean Acid Metabolism in Talinum triangulare.

A chromosome-scale genome assembly of Hordeum erectifolium: genomic, transcriptomic and anatomical adaptations to drought in a wild barley relative

A prototypic ARC that implements all specification standards accordingly

Assembly and Quantification of Co-Cultures Combining Heterotrophic Yeast with Phototrophic Sugar-Secreting Cyanobacteria

T7 RNA polymerase-based gene expression from a transcriptionally silent rDNA spacer in the endosymbiont-harboring trypanosomatid Angomonas deanei

Live-cell imaging enables reporter-free monitoring of the circadian rhythm in individual Synechocystis cells

Lipidomics analysis of bacterial acyltransferase chiral substrate specificity

A template ARC for transcriptomics RNA-Seq generated data.

This is an updated and slimmed-down version of a published ARC (https://doi.org/10.5447/ipk/2025/3) to showcase how to represent MIAPPE v1.2 in the ARC framework.

In seed plants, a tethering complex of two proteins, SEED LIPID DROPLET PROTEIN (SLDP) and LIPID DROPLET PLASMA MEMBRANE ADAPTOR (LIPA), binds lipid droplets (LDs) to the plasma membrane (PM). While the physiological function remains obscure, the conservation of the process is strong. The tethering of LDs can be observed broadly in a variety of seed plants seedling tissues. Interestingly a tethering might not be limited to the PM and could be associated with other organelles in other plants. Here, we show that LIPA and SLDP have emerged in the early period of land plant evolution likely before the emerging seed plants became dominant. They are, however, most prominently conserved in seed plants and their homologs share the tethering function in ectopic expression systems. This interaction is robust and can even be functional across taxon boundaries. The evolution of a LD PM tether in that significant time point also puts the LD in focus of land plant evolution. The involvement of LDs in conquest of terrestrial ecosystems and the studying thereof can give valuable insight in physiological processes that made early terrestrialization events possible. Further studies need to evaluate the function of the discovered homologs in non-seed plants.

This project characterizes the genomic integrity of Escherichia coli K-12 through a comprehensive workflow spanning bacterial cultivation, DNA extraction, library preparation, Illumina sequencing, and bioinformatic analysis. The integrated approach ensures high-quality genome assemblies consistent with the reference strain.