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Live-cell imaging enables reporter-free monitoring of the circadian rhythm in individual Synechocystis cells

This project characterizes the genomic integrity of Escherichia coli K-12 through a comprehensive workflow spanning bacterial cultivation, DNA extraction, library preparation, Illumina sequencing, and bioinformatic analysis. The integrated approach ensures high-quality genome assemblies consistent with the reference strain.

The amplitude between the minimum and the maximum ambient temperature a plant has to endure during its life cycle is much larger in terrestrial than in aquatic environments. As such, especially elevated ambient temperatures are among the most prominent abiotic factors plants had to cope with to successfully colonize land. It is known that the phenotypic plasticity in eudicot land plants allows them to adjust shoot architecture to acclimate and adapt to elevated temperatures. How and when this mechanism, thermomorphogenesis, has originated is unknown. Likewise, we do not know whether bryophytes, whose body plans is probably very similar to that of the first terrestrial plants, perform thermomorphogenesis. In this project, we will therefore characterize the response of bryophytes (Physcomitrium patens and Marchantia polymorpha) to elevated temperatures on both the morphological as well as the physiological level, including elucidation of temperature sensitive signaling cascades. In addition to comparative analyses we will also follow an unbiased approach, primarily using the liverwort Marchantia polymorpha. To identify signaling and response modules, we will generate high density transcriptomic and phosphoproteomic datasets and use them to construct gene regulatory networks based on machine learning approaches. As such, we aim to combine morphological and molecular data in this project to better understand how bryophytes cope with elevated ambient temperatures. With this knowledge. we might then be in a position to design experiments that directly address the emergence of such mechanisms and their role during the terrestrialization of land by plants.

ARC for the SNPstar project describing the structure of the input and output data of the Snakemake workflow used for setting up a SNPstar instance.

Chromosome-scale Solanum pennellii and Solanum cheesmaniae genome assemblies reveal structural variants, repeat content and recombination barriers of the tomato clade

Fungi hijack a ubiquitous plant apoplastic endoglucanase to release a ROS scavenging β-glucan decasaccharide to subvert immune responses

A GH81-type β-glucan-binding protein enhances colonization by mutualistic fungi in barley

PPD-H1 Improves Stress Resistance and Energy Metabolism to Boost Spike Fertility under High Ambient Temperatures

Algae cultures were grown mixotrophically (TAP). After 24h of 35°C/40°C the cells were shifted back to room temperature for 48h. 'omics samples were taken.

Reversible Burst of Transcriptional Changes during Induction of Crassulacean Acid Metabolism in Talinum triangulare.

A chromosome-scale genome assembly of Hordeum erectifolium: genomic, transcriptomic and anatomical adaptations to drought in a wild barley relative