Explore ARCs
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Sugar transporters spatially organize microbiota colonization along the longitudinal root axis of Arabidopsis
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Predmoter — cross-species prediction of plant promoter and enhancer regions
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The trace element iron is essential for life, but elevated levels can rapidly cause cellular damage through oxidative stress. Bacteria, like Corynebacterium glutamicum, tightly regulate iron and heme homeostasis via the global regulators DtxR and HrrA. This study provides the first analysis of the genome-wide binding patterns of these two regulators demonstrating significant differences in binding dependent on the tested iron regimes. Overall, we identified 25 new DtxR targets and 210 previously unknown HrrA targets, including genes with crucial roles in central metabolism and DNA repair. Notably, DtxR was shown to link iron metabolism to methionine synthesis, which might be important to protect the cell from oxidative stress. Our findings highlight the interconnected nature of DtxR and HrrA networks and underscore the value of condition-specific analysis to deepen the understanding of how bacteria adapt to environmental changes.
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Exploring natural genetic variation in photosynthesis-related traits of barley in the field
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Traits linked to natural variation of sulfur content in Arabidopsis thaliana
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Reversible Burst of Transcriptional Changes during Induction of Crassulacean Acid Metabolism in Talinum triangulare.
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A systematic overexpression approach reveals native targets to increase squalene production in Synechocystis sp. PCC 6803
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A GH81-type β-glucan-binding protein enhances colonization by mutualistic fungi in barley
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Crassulacean acid metabolism (CAM) has evolved as a water-saving strategy, and its engineering into crops offers an opportunity to improve their water use efficiency. This requires a comprehensive understanding of the regulation of the CAM pathway. Here, we use the facultative CAM species Talinum triangulare as a model in which CAM can be induced rapidly by exogenous abscisic acid. RNA sequencing and metabolite measurements were employed to analyse the changes underlying CAM induction and identify potential CAM regulators. Non-negative matrix factorization followed by k-means clustering identified an early CAM-specific cluster and a late one, which was specific for the early light phase. Enrichment analysis revealed abscisic acid metabolism, WRKY-regulated transcription, sugar and nutrient transport, and protein degradation in these clusters. Activation of the CAM pathway was supported by up-regulation of phosphoenolpyruvate carboxylase, cytosolic and chloroplastic malic enzymes, and several transport proteins, as well as by increased end-of-night titratable acidity and malate accumulation. The transcription factors HSFA2, NF-YA9, and JMJ27 were identified as candidate regulators of CAM induction. With this study we promote the model species T. triangulare, in which CAM can be induced in a controlled way, enabling further deciphering of CAM regulation.
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Fungi hijack a ubiquitous plant apoplastic endoglucanase to release a ROS scavenging β-glucan decasaccharide to subvert immune responses
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Kinetic data for modeling the dynamics of the enzymes involved in animal fatty acid synthesis
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Host preference and invasiveness of commensal bacteria in the Lotus and Arabidopsis root microbiota
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Cofunctioning of bacterial exometabolites drives root microbiota establishment
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early maturity 7 controls photoperiodic flowering in barley by modifying the diurnal expression pattern of the major photoperiod response gene Ppd-H1.
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