Figure 6 B) caption
Figure 6 B). Schematic representation of different forms of CWAs in barley roots in response to fungal colonization. These include papillae formation at the sites of attempted (1) and successful (2) hyphal penetration, as well as an extensive reaction along the CWs (3).
Figure 6 B) source
Supplementary Figure 3 A) caption
Supplementary Figure 3 A). Example of R. irregularis structures in barley roots at 28 dpi. R. irregularis structures were stained with 5% ink (Pelikan) and fungal structures were observed using a light microscope (AxioStar, Carl Zeiss, Jena, Germany) at 10X magnification.
Supplementary Figure 3 A) source
https://www.cell.com/current-biology/pdfExtended/S0960-9822(23)01449-5
Supplementary Figure 3 B) caption
Supplementary Figure 3 B). The control line and gbp1 gbp2 mutants were inoculated with R. irregularis spores and roots were harvested at 28 dpi. Barley roots were analyzed at 300 randomly chosen sections (covering 30 cm of root length) for the presence of R. irregularis structures including arbuscules, vesicles, intraradical hyphae (IRH), and extraradical hyphae (ERH). All data points are plotted on graphs as circles (n = 4). Boxplot elements in this figure: center line, median; box limits, upper and lower quartiles; whiskers, 1.5 × interquartile range. Different letters represent statistically significant differences based on one-way ANOVA and Tukey’s post hoc test (significance threshold: p ≤0.05).
Supplementary Figure 3 B) source
https://www.cell.com/current-biology/pdfExtended/S0960-9822(23)01449-5
Supplementary Figure 3 C) caption
Supplementary Figure 3 C). Images of barley control and gbp mutant line seedlings during B. sorokiniana colonization. Barley control and gbp mutant lines were inoculated with B. sorokiniana and grown in jars under axenic conditions for 7 days. Images were taken at 7 dpi to assess any shoot or root phenotypes of the gbp mutants (scale bar = 3 cm). Mock-inoculated images can be found in Figure S2B.
Supplementary Figure 3 C) source
https://www.cell.com/current-biology/pdfExtended/S0960-9822(23)01449-5
Supplementary Figure 3 D) caption
Supplementary Figure 3 D). Representative images of B. hordei infection structures on barley leaves. Barley leaves colonized by B. hordei were analyzed for penetration success using bright field microscopy (scale bar = 20 µm). Fungal structures were stained using Coomassie brilliant blue, and secondary hyphae formation was assessed from 50 germinated conidia spores in both the tip and middle area of each leaf
Supplementary Figure 3 D) source
https://www.cell.com/current-biology/pdfExtended/S0960-9822(23)01449-5