Facultative CAM in Talinum

Data origin

• see isa.investigation.xlsx for details
• Adapted from

  Brilhaus, D., Bräutigam, A., Mettler-Altmann, T., Winter, K., and Weber, A.P.M. (2016). Reversible Burst of Transcriptional Changes during Induction of Crassulacean Acid Metabolism in Talinum triangulare. Plant Physiology 170: 102–122. https://doi.org/10.1104/pp.15.01076

• studies/TalinumGenomeDraft originates from https://git.nfdi4plants.org/hhu-plant-biochemistry/talinum-fruticosum-genome

Facultative CAM in Talinum triangulare

Description

Drought tolerance is a key factor for agriculture in the 21st century as it is a major determinant of plant survival in natural ecosystems as well as crop productivity. Plants have evolved a range of mechanisms to cope with drought, including a specialized type of photosynthesis termed Crassulacean acid metabolism (CAM). CAM is associated with stomatal closure during the day as atmospheric CO2 is assimilated primarily during the night, thus reducing transpirational water loss. The tropical herbaceous perennial species Talinum triangulare is capable of transitioning, in a facultative, reversible manner, from C3 photosynthesis to weakly expressed CAM in response to drought stress. The transcriptional regulation of this transition has been studied. Combining mRNA-Seq with targeted metabolite measurements, we found highly elevated levels of CAM-cycle enzyme transcripts and their metabolic products in T. triangulare leaves upon water deprivation. The carbohydrate metabolism is rewired to reduce the use of reserves for growth to support the CAM-cycle and the synthesis of compatible solutes. This large-scale expression dataset of drought-induced CAM demonstrates transcriptional regulation of the C3–CAM transition. We identified candidate transcription factors to mediate this photosynthetic plasticity, which may contribute in the future to the design of more drought-tolerant crops via engineered CAM.

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Relationships between Assays and Studies

---
title: Facultative-CAM-in-Talinum
---
graph TB
TalinumGenomeDraft_study["TalinumGenomeDraft"];
TalinumFacultativeCAM_study["TalinumFacultativeCAM"];
MassHunter_targets_assay["MassHunter_targets"];
Talinum_RNASeq_minimal_assay["Talinum_RNASeq_minimal"];
GCqTOF_targets_assay["GCqTOF_targets"];
TalinumFacultativeCAM_study --> Talinum_RNASeq_minimal_assay;
TalinumFacultativeCAM_study --> GCqTOF_targets_assay ;
GCqTOF_targets_assay --> MassHunter_targets_assay 
class TalinumGenomeDraft_study,TalinumFacultativeCAM_study study;
class MassHunter_targets_assay,Talinum_RNASeq_minimal_assay,GCqTOF_targets_assay assay;
classDef assay fill:#4FB3D9,stroke:#333,stroke-width:2px,color:#3A3A3A;
classDef study fill:#B4CE82,stroke:#333,stroke-width:2px,color:#3A3A3A;

Figure 1:This flowchart highlights the relationship between assays (highlighted in blue) and studies (highlighted in green)

Additional details

Table of Contents

1. Contacts

2. Publication

3. Studies

   • TalinumGenomeDraft
   • TalinumFacultativeCAM

4. Assays

   • MassHunter_targets
   • Talinum_RNASeq_minimal
   • GCqTOF_targets

5. References

Contacts

Publication

Study : TalinumGenomeDraft

Description

tba

Additional details

Annotation headers

Study : TalinumFacultativeCAM

Description

tba

Additional details

Annotation headers

Characteristics: organism,organism part,plant age

Parameters: growth day length,light intensity exposure,humidity day,temperature day,temperature night

Factors: watering exposure,Timepoint,timepoint-ZT,Photosynthesis mode

Assay : MassHunter_targets

Description

Drought tolerance is a key factor for agriculture in the 21st century as it is a major determinant of plant survival in natural ecosystems as well as crop productivity. Plants have evolved a range of mechanisms to cope with drought, including a specialized type of photosynthesis termed Crassulacean acid metabolism (CAM). CAM is associated with stomatal closure during the day as atmospheric CO2 is assimilated primarily during the night, thus reducing transpirational water loss. The tropical herbaceous perennial species Talinum triangulare is capable of transitioning, in a facultative, reversible manner, from C3 photosynthesis to weakly expressed CAM in response to drought stress. The transcriptional regulation of this transition has been studied. Combining mRNA-Seq with targeted metabolite measurements, we found highly elevated levels of CAM-cycle enzyme transcripts and their metabolic products in T. triangulare leaves upon water deprivation. The carbohydrate metabolism is rewired to reduce the use of reserves for growth to support the CAM-cycle and the synthesis of compatible solutes. This large-scale expression dataset of drought-induced CAM demonstrates transcriptional regulation of the C3–CAM transition. We identified candidate transcription factors to mediate this photosynthetic plasticity, which may contribute in the future to the design of more drought-tolerant crops via engineered CAM.

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Additional details

Annotation headers

Assay : Talinum_RNASeq_minimal

Description

Drought tolerance is a key factor for agriculture in the 21st century as it is a major determinant of plant survival in natural ecosystems as well as crop productivity. Plants have evolved a range of mechanisms to cope with drought, including a specialized type of photosynthesis termed Crassulacean acid metabolism (CAM). CAM is associated with stomatal closure during the day as atmospheric CO2 is assimilated primarily during the night, thus reducing transpirational water loss. The tropical herbaceous perennial species Talinum triangulare is capable of transitioning, in a facultative, reversible manner, from C3 photosynthesis to weakly expressed CAM in response to drought stress. The transcriptional regulation of this transition has been studied. Combining mRNA-Seq with targeted metabolite measurements, we found highly elevated levels of CAM-cycle enzyme transcripts and their metabolic products in T. triangulare leaves upon water deprivation. The carbohydrate metabolism is rewired to reduce the use of reserves for growth to support the CAM-cycle and the synthesis of compatible solutes. This large-scale expression dataset of drought-induced CAM demonstrates transcriptional regulation of the C3–CAM transition. We identified candidate transcription factors to mediate this photosynthetic plasticity, which may contribute in the future to the design of more drought-tolerant crops via engineered CAM.

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Additional details

Annotation headers

Parameters: biosource amount,extraction method,extraction buffer,extraction buffer volume,RNA quality check,library strategy,library selection,library layout,library preparation kit,library preparation kit version,adapter sequence,next generation sequencing instrument model,base-calling software,base-calling software version,Raw data file format

Assay : GCqTOF_targets

Description

Drought tolerance is a key factor for agriculture in the 21st century as it is a major determinant of plant survival in natural ecosystems as well as crop productivity. Plants have evolved a range of mechanisms to cope with drought, including a specialized type of photosynthesis termed Crassulacean acid metabolism (CAM). CAM is associated with stomatal closure during the day as atmospheric CO2 is assimilated primarily during the night, thus reducing transpirational water loss. The tropical herbaceous perennial species Talinum triangulare is capable of transitioning, in a facultative, reversible manner, from C3 photosynthesis to weakly expressed CAM in response to drought stress. The transcriptional regulation of this transition has been studied. Combining mRNA-Seq with targeted metabolite measurements, we found highly elevated levels of CAM-cycle enzyme transcripts and their metabolic products in T. triangulare leaves upon water deprivation. The carbohydrate metabolism is rewired to reduce the use of reserves for growth to support the CAM-cycle and the synthesis of compatible solutes. This large-scale expression dataset of drought-induced CAM demonstrates transcriptional regulation of the C3–CAM transition. We identified candidate transcription factors to mediate this photosynthetic plasticity, which may contribute in the future to the design of more drought-tolerant crops via engineered CAM.

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Additional details

Annotation headers

Parameters: Bio entity,Biosource amount,Biosource material state,Extraction buffer,Extraction buffer volume,Internal standard,Sample volume,MS sample post-extraction,MS sample resuspension,MS derivatization,MS sample type,Chromatography instrument model,Chromatography autosampler model,Chromatography column type,Chromatography column model,mobile phase,Chromatography injection volume,Chromatography injection mode,Chromatography gradient,scan polarity,scan window lower limit,scan window upper limit,scan rate,instrument model,ionization type,mass analyzer type,detector type

References

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