Skip to content
Snippets Groups Projects

[curation] link figures and movies in assay READMEs

Merged Dominik Brilhaus requested to merge ceplas-db into main
1 file
+ 9
1
Compare changes
  • Side-by-side
  • Inline
# Root Length Measurement
## Movie S1. Arabidopsis seedlings grown in a RoPod chamber have normal phenotype.
The time-lapse movie of root growth illustrated and quantified in the Fig.1g-i. Seed germination and
root elongation of seedling grown in the RoPod v24.3 are comparable to the growth under or on
top of the growth medium in a Petri plate. Additionally, similar growth of wild-type WT) plants
corroborates that RoPod provides favourable growth conditions for Arabidopsis plants. Images
were acquired using SPIRO.
![41598_2024_63226_MOESM1_ESM.mp4](assays/RootLengthMeasurement/dataset/41598_2024_63226_MOESM1_ESM.mp4)
## Figure 1g-i
RoPod, a toolkit comprising plant growth protocol and microscopy chamber optimized for long-term time-lapse imaging of Arabidopsis roots.
(g–i) Growth conditions in RoPod chambers are equivalent to standard growing conditions on Petri plates, as demonstrated by comparing root growth on the top of the medium, under the medium inside the standard Petri plate and in RoPod chambers. Seeds of wild-type (WT) and autophagy-deficient atg5-1 Arabidopsis plants expressing GFP-ATG8 were sown on the agar medium in Petri plates or RoPods. Plates and chambers were placed on SPIRO under long day conditions and imaged for a week. (h) Zoomed-in insets shown in (g). (i) The chart represents data from two independent experiments. The root lengths were measured on the 7th day after seed plating. One-Way ANOVA test revealed no difference in root length of seedlings for the WT seedlings grown in RoPods and on Petri plates, significance level of 0.05, n = 103.
![Figure1g-i.png](dataset/Figure1g-i.png)
Loading