Figure 6 A caption
Figure 6 A). Barley root CW responses of control and gbp1 gbp2 mutant lines colonized by S. indica. Samples were fluorescently labeled with concanavalin A (Con A-AF633, cyan) and wheat germ agglutinin (WGA-AF488, green) for visualization of CWAs and fungal structures, respectively, then analyzed by confocal laser scanning microscopy.
Figure 6 A) source
Supplementary Figure 6 A) caption
Supplementary Figure 6 A). The number and size of papillae upon S. indica colonization does not differ between the control and gbp1 gbp2 mutant lines. S. indica-colonized roots of the barley control line and gbp1 gbp2 mutant lines were stained with concanavalin A (ConA-AF633, cyan) and wheat germ agglutinin (WGA-AF488, green) for visualization of papillae and fungal structures, respectively. Images were acquired using a confocal microscope. The diameter of papillae found in the control and gbp1 gbp2 mutant lines colonized by S. indica (n = 61, upper boxplot). The number of papillae were quantified in the control line and gbp1 gbp2 mutant lines colonized by S. indica (n = 25, lower boxplot). Different letters represent statistically significant differences based on one-way ANOVA (significance threshold: p ≤0.05).
Supplementary Figure 6 A) source
https://www.cell.com/current-biology/pdfExtended/S0960-9822(23)01449-5
Supplementary Figure 6 B) caption
Supplementary Figure 6 B). The barley control and gbp1 gbp2 mutant lines do not form spontaneous papillae or CW responses in absence of fungal colonization. Mock-treated roots of the control and gbp1 gbp2 mutant lines were stained with concanavalin A (ConA-AF633, cyan) and wheat germ agglutinin (WGAAF488, green) for visualization of CWAs and fungal structures, respectively. Images were acquired using a confocal microscope.
Supplementary Figure 6 B) source
https://www.cell.com/current-biology/pdfExtended/S0960-9822(23)01449-5
Supplementary Figure 6 C) caption
Supplementary Figure 6 C). Aniline blue stained callose deposition differs in control and gbp1 gbp2 mutant lines during S. indica colonization. Barley control and gbp1 gbp2 mutant lines were inoculated with sterile water (mock) or S. indica and grown under axenic conditions. Root samples were harvested at 6 dpi and stained with aniline blue to visualize callose deposition. Images were acquired with a confocal microscope. ConA, concanavalin A; CW(A), cell wall (appositions); WGA, wheat germ agglutinin.
Supplementary Figure 6 C) source
https://www.cell.com/current-biology/pdfExtended/S0960-9822(23)01449-5