Allelism test
Complementation crosses were performed to confirm that lwd1 and eam7 are allelic, which are both recessive mutations. For this, homozygous lwd1 mutants lwd1-26, lwd1-390, and lwd1-402 were crossed with BW(Ppd-H1, eam7). F1 plants were grown under SD conditions and flowering time was scored. If lwd1 and eam7 are allelic, we expected the F1 plants to display the mutant phenotype and be early flowering under SD.
Study overview:
parent plants (lwd1 mutants, BW(Ppd-H1,eam7), BW(Ppd-H1), GP-fast)
-> assay: complementation_crosses
output: F1 plants
-> assay: plant_genotyping
output: F1 genotype information
-> assay: plant_phenotyping
output: F1 flowering data
Results can be found in Figure 6 and Supplemental Figure S8.