Physiology, biochemistry and anatomy of young fully developed leaves from Brassicaceae species with C3 and C3-C4 (C2) photosynthesis
Aim of study:
Identification of physiological, biochemical and anatomical features related to the C3-C4 photosynthesis phenotype in the Brassicaceae
Species/taxa: C3-C4 phenotype in bold, C. gynandra is C4 phenotype, all others with C3 phenotype
- Arabidopsis thaliana (L.) Heynh. (At),
- Brassica gravinae Ten. (4 accessions, Bg1, Bg2, Bg3 and Bg4),
- Brassica juncea (L.) Czern. (Bj),
- Brassica napus L. (Bn),
- Brassica nigra (L.) W.D.J. Koch subsp.nigra va nigra (Bni),
- Brassica oleraceae L. (Bo),
- Brassica rapa L. (Br),
- Brassica repanda (Willd.) (Be),
- Brassica tournefortii Gouan. (2 accessions, Bt1 and Bt2),
- Carrichtera annua (L.) DC. (Ca),
- Diplotaxis acris Boiss. (Da),
- Diplotaxis erucoides (L.) DC. (De),
- Diplotaxis harra Boiss. (Dh),
- Diplotaxis muralis (L.) DC. (Dm),
- Diplotaxis tenuifolia (L.) DC. (Dt),
- Diplotaxis tenuisiliqua Delile (Ds),
- Diplotaxis viminea (L.) DC. (Dv),
- Eruca sativa Mill. (Es),
- Hirschfeldia incana (L.) Lagr.-Foss (2 accessions HIR1 and HIR3),
- Moricandia arvensis (L.) DC. (Ma),
- Moricandia moricandioides (Boiss.) Heywood (Mm),
- Moricandia nitens E.Durand & Barratte (Mn),
- Moricandia sinaica Boiss. (Msi),
- Moricandia spinosa Pomel (Mp),
- Moricandia suffruticosa (Desf.) Coss. & Durieu(Ms),
- Raphanus raphanistrum L. (Rr),
- Raphanus sativus subsp sativus (L.) (Rs)
- Sinapis alba L. (Sa)
-
Gynandropsis gynandra (L.) Briq. /Cleome gynanadra L.
Analysis/measurements:
- gas exchange by Li6800 IRGA measuring A-ci response and calculation of CO2 compensation point
- primary metabolite pattern by GCMS
- element analysis (CN ratio, 13C) by EA-IRMS
- vein density (vein length per area)
- PEPC activity in leaf extracts by spectrophotometry
- Specific leaf area (area per dry weight)
- analysis of leaf cross sections by light microscopy and determination of organelle area in bundle sheath facing vein or mesopyll/intercellular space
Data presentation: This ARC contains the data measured per single plant and the raw data for the GCMS and EA-IRMS analysis.
Publication:
Results and discussion of the presented data has been published in the following paper:
Schlüter, U., Bouvier, J. W., Guerreiro, R., Malisic, M., Kontny, C., Westhoff, P., Stich, B. & Weber, A.
P. M. (2023), Brassicaceae display diverse photorespiratory carbon recapturing mechanisms, Journal of Experimental Botany, https://doi.org/10.1093/jxb/erad250
Analysis of the genomes of the selected species/taxa can be found in the following paper:
Guerreiro R, Bonthala VS, Schlüter U, Triesch S, Weber APM, Stich B. 2023. A genomic panel for studying C3–C4 intermediate photosynthesis in the Brassiceae tribe. Plant, Cell & Environment https://doi.org/10.1111/pce.14662.
Analysis of the GLDP1 promoter and the potential involvement of transposible elements in evolution of C3-C4 phenotype in the Brassicaceae is discussed in:
Triesch S, Denton AK, Buchmannn JP, Reichel-Deland V, Guerreiro R, Schlüter U, Weber APM. 2023. Transposable elements contribute to the establishment of the glycine shuttle in Brassicaceae species. doi: https://doi.org/10.1101/2022.12.06.519256