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  • hhu-plant-biochemistry/Wrobel-2023-CastorBeanEndospermProteome
  • ceplas/Wrobel-2023-CastorBeanEndospermProteome
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## Ricinus-communis-seeds
![fpls-14-1182105-g001.jpg](resources/fpls-14-1182105-g001.jpg)
**Figure 1**
Endosperm morphology in seeds and germinating seedling of R. communis. Photographs were taken from the whole plant (upper panel) and two cotyledons embedded in the endosperm (lower panel) from mature seeds (dry), 24h-imbibed seeds (imb) and from 1- to 7-day old dark-grown castor bean seedlings (1-7d). Scale bar = 1 cm.
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# 2.1 Plant growth conditions
Dry seeds of Ricinus communis var. zanzibariensis were surface- sterilized in a solution of 0.1% (w/v) 8-quinolinol in water for ten minutes and soaked in running tap water over night for seed imbibition. The imbibed seeds were placed on moist vermiculite and incubated at 30°C in the dark (Beevers and Breidenbach, 1974).
Dry seeds of *Ricinus communis* var. *zanzibariensis* were surface- sterilized in a solution of 0.1% (w/v) 8-quinolinol in water for ten minutes and soaked in running tap water over night for seed imbibition. The imbibed seeds were placed on moist vermiculite and incubated at 30°C in the dark (Beevers and Breidenbach, 1974).
- Beevers, H., and Breidenbach, R. W. (1974). “Glyoxysomes,” in Methods in enzymology, Vol 16 Biomembranes, Part A. Eds. S. Fleciher and L. packer (New York London: Academic Press), 565–571.
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studies/Ricinus-communis-seeds/resources/fpls-14-1182105-g001.jpg

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