Carbon Availability Transcriptomics

Chlamydomonas reinhardtii CC-1690 was grown in bioreactors. When the cells reached a non-stationary density, the acetate supply was stopped and the medium was kept at 25°C (control) or heated to 35 °C and 40 °C. Additionally to a preheat sample, four further samples were taken as triplicates after 2h, 4h, 8h, and 24h of heat treatment (nopump samples). The samples were analysed with NGS. In Zhang et al 2022 samples were taken at the same time points for 35°C and 40°C but with constant nutrient supply (TAP samples).

Table 1: Sampling schema for Transcriptomics analysis. Three biological replicates were measured.