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Commit a40d5b5e authored by Daniel Heß's avatar Daniel Heß
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test2

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TABLE 1 Oligonucleotides used in this study
ID Sequence Description
CBr-CABconsensus-F TAATACGACTCACTATAGGGTAGGCCCAGGCGTTGTT Northern Blot
T7-CBr-CABconsensus-R GACCGCCCCAAGTACC Northern Blot
TruSeq_Sense_nodal AATGATACGGCGACCACCGAGATCTACAC-CAAGTGGGACACTCTTTCCCTACACGACGCTCTTCCGATCT TruSeq_Sense_primer i5 Barcode
TruSeq_Sense_control AATGATACGGCGACCACCGAGATCTACAC-AGATTTGGACACTCTTTCCCTACACGACGCTCTTCCGATCT TruSeq_Sense_primer i5 Barcode
TruSeq_Antisense_nodal CAAGCAGAAGACGGCATACGAGAT-TCCGCGAAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT TruSeq_Antisense_primer i7 Index
TruSeq_Antisense_control CAAGCAGAAGACGGCATACGAGAT-TCCGCGAAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT TruSeq_Antisense_primer i7 Index
Note: An added T7 promoter sequence is highlighted in boldface letters.
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Input: amino acid sequences aligned with M-coffee
BEAST version 2.7.3
BEAUTi settings:
Gamma Site Model, Substitution Model Blosum62
Strict Clock - mean clock rate 1.0
Prior Yule model, uniform (0,0,infinity), initial [1.0][0.0,infinite]
MCMC chain length 1e6, store every 1e4, no pre Burnin, num init attempts 10
Tracer v1.7.2:
validate ESS > 200
TreeAnnotator:
maximum clade credibility tree
burnIn 50%
posterior probability limit 0.5 (median node heights)
FigTree v1.4.4:
visualisation of generated dendrograms
- display tip labels, node labels (posterior)
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workflows/RNAseqWorkflow_Data.png

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