studies/Plant Growth Conditions/protocols/Flowpot.md

+## Flowpot
+
+Flowpots were assembled and inoculated as described below. Each Flowpot was first flushed with 50 ml sterile MiliQ water and then 50 ml half strength Murashige and Skoog medium with vitamins (1⁄2 MS; 2.2 g/l, Duchefa Biochemie, 0.5 g/l MES, pH 5.7) containing the bacterial inoculum. Per Flowpot, five surface-sterilized and stratified *A. thaliana* Col-0 seeds were pipetted. Microboxes were then incubated in a light cabinet under short day conditions (10 h light at 21 °C, 14 h dark at 19 °C) for 14 days and randomized every 2–3 days.
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studies/Plant Growth Conditions/protocols/PlantGrowthConditions.md

+## Plant Growth Conditions
+
+
+A. thaliana Col-0 wild-type (N60000) was obtained from the Nottingham Arabidopsis Stock Centre (NASC). *Arabidopsis thaliana* Col-0 seeds were sterilized using 70% ethanol and bleach. Seeds were submerged in 70% ethanol and left shaking at 40 rpm for 14 minutes. Ethanol was removed before the seeds were submerged in 8.3% sodium hypochlorite (Roth) containing 1 μl of Tween 20 (Sigma-Aldrich) and left shaking at 40 rpm for 4 minutes. Under sterile conditions, the seeds were washed 7x times and finally taken up with sterile 10 mM MgC12. Seeds were left for stratification at 4 °C for 3 days. Seed sterility was confirmed by plating approx. 100 seeds on a 50% TSA plate.
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