01_plant_material.md

Note: 
- This is a somewhat polished protocol as found in a publication. 
- This is not an example for a typical lab protocol or method. 

Plant Material and Growth Conditions

Talinum triangulare plants were grown in Miracle-Gro Potting Mix (Miracle-Gro) in “Short-One” treepots, 1.6 l (Stuewe and Sons). The experiment was initiated with 28-d-old plants in a controlled environment chamber (Environmental Growth Chambers) maintained under 12 h light (30°C, 37% relative humidity)/12 h dark (22°C) cycles. Photon flux density at leaf level was 425 mmol m^-2 s^-1. Irrigation was withheld on day 1 and recommenced on day 14. Leaves were harvested when plants were well-watered as well as after 4, 9, and 12 days of water deprivation and watered for two days following the drought period.