Skip to content
Snippets Groups Projects
Commit 1a41155e authored by Dominik Brilhaus's avatar Dominik Brilhaus
Browse files

move deseq readme to cwl docs section

parent bd465bfd
No related branches found
No related tags found
1 merge request!18Docker dependencies
Pipeline #9434 failed
# DESeq2
Workflow used for **differential gene expression analysis**
## DESeq2 docs
- https://bioconductor.org/packages/release/bioc/html/DESeq2.html
## Importing kallisto output with tximport
- https://bioconductor.org/packages/release/bioc/vignettes/tximport/inst/doc/tximport.html#kallisto
## Run pure script (to test)
### Install R dependencies for deseq2
```R
if (!require("BiocManager", quietly = TRUE))
install.packages("BiocManager")
BiocManager::install("DESeq2")
library("DESeq2")
BiocManager::install("tximport")
library("tximport")
BiocManager::install("rhdf5")
library("rhdf5")
```
## Multi-package containers
- R and combinations of library dependencies are available as multi-package containers from [BioContainers](https://github.com/BioContainers/multi-package-containers)
- Searched for `repo:BioContainers/multi-package-containers deseq2 tximport rhdf5`
- and found `quay.io/biocontainers/mulled-v2-05fd88b9ac812a9149da2f2d881d62f01cc49835:a10f0e3a7a70fc45494f8781d33901086d2214d0-0` :tada:
#!/usr/bin/env cwl-runner
doc: |
DESeq2 example workflow
DESeq2 example workflow for **differential gene expression analysis**
This workflow runs DESeq2 on the output of the kallisto workflow
and the metadata file.
It runs an R script, deseq2.R, which ideally should be split into three sub scripts and accordingly three workflow steps
......@@ -9,6 +10,17 @@ doc: |
2. Prep / run deseq2
3. Plot results
## DESeq2 docs:
https://bioconductor.org/packages/release/bioc/html/DESeq2.html
## Importing kallisto output with tximport
https://bioconductor.org/packages/release/bioc/vignettes/tximport/inst/doc/tximport.html#kallisto
## Multi-package containers
- R and combinations of library dependencies are available as multi-package containers from [BioContainers](https://github.com/BioContainers/multi-package-containers)
- Searched for `repo:BioContainers/multi-package-containers deseq2 tximport rhdf5`
- and found `quay.io/biocontainers/mulled-v2-05fd88b9ac812a9149da2f2d881d62f01cc49835:a10f0e3a7a70fc45494f8781d33901086d2214d0-0` :tada:
cwlVersion: v1.2
class: CommandLineTool
hints:
......
0% Loading or .
You are about to add 0 people to the discussion. Proceed with caution.
Finish editing this message first!
Please register or to comment