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Commit df82c08c authored by Dominik Brilhaus's avatar Dominik Brilhaus
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rm upgrade README

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...@@ -38,4 +38,4 @@ assays/Talinum_RNASeq_minimal/isa.assay.xlsx | "RNA extraction" v. 1.1.6<br>"RNA ...@@ -38,4 +38,4 @@ assays/Talinum_RNASeq_minimal/isa.assay.xlsx | "RNA extraction" v. 1.1.6<br>"RNA
Directory | Purpose Directory | Purpose
---- | ---- ---- | ----
[_GEO_submission](./_GEO_submission) | Example metadata files as required for submission to GEO [_GEO_submission](./_GEO_submission) | Example metadata files as required for submission to GEO
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## Notes and ToDos
### Experimental metadata in isa.assay.xlsx
- split GEO SWATE templates into four sheets
- 1SPL01_plants
- 2EXT01_RNA
- 3ASY01_RNASeq
- 4COM01_RNASeq
### Adding large raw data via git lfs
1. Before adding the files to the ARC, track them via `git lfs`
```bash
git lfs track "01_kallisto_index"
```
1. Move / add the large data files to the respective folders
1. Add them via `git add`
```bash
git add runs/run1/01_kallisto_index
```
1. Commit
## Bumping to ARC v1.1 (23.03.2022)
### by arcCommander / shell
> depends on arcCommander v3 or higher
```bash
arc a list
arc study unregister -s TalinumFacultativeCAM # unregister old study version
arc study add -s TalinumFacultativeCAM # add fresh
mv TalinumFacultativeCAM.study.xlsx studies/TalinumFacultativeCAM/old.study.xlsx # move old study metadata to new study
arc study add -s TalinumGenomeDraft # add draft genome as study
mv externals/Talinum.gm.CDS.nt.fa studies/TalinumGenomeDraft/resources # mv genome to resources
rm -r externals
rm inv.json
arc assay register -s TalinumFacultativeCAM -a Talinum_RNASeq_minimal # re-register assay
arc update
mv assays/Talinum_RNASeq_minimal/protocols/01_plant_material.md studies/TalinumFacultativeCAM/protocols # move plant growth from assay to study
arc assay remove -s TalinumFacultativeCAM -a RNASeq_Kallisto_quant # add computational parts as assay
```
### by hand
- move plant growth sheet isa.assay to isa.study
- remove computational RNASeq sheet from assays as it partly duplicates the (not yet CWLed) kallisto workflow
### add placeholder sample descriptors
```bash
arc_root=$(pwd)
cd studies/TalinumFacultativeCAM/resources/
touch \
DB_097 \
DB_099 \
DB_103 \
DB_161 \
DB_163 \
DB_165
cd $arc_root
```
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