Physiology, biochemistry and anatomy of young fully developed leaves from Brassicaceae species with C₃ and C₃-C₄ (C₂) photosynthesis

Aim of study:
Identification of physiological, biochemical and anatomical features related to the C₃-C₄ photosynthesis phenotype in the Brassicaceae

Species/taxa: C₃-C₄ phenotype in bold, C. gynandra is C₄ phenotype, all others with C₃ phenotype

• Arabidopsis thaliana (L.) Heynh. (At),
• Brassica gravinae Ten. (4 accessions, Bg1, Bg2, Bg3 and Bg4),
• Brassica juncea (L.) Czern. (Bj),
• Brassica napus L. (Bn),
• Brassica nigra (L.) W.D.J. Koch subsp.nigra va nigra (Bni),
• Brassica oleraceae L. (Bo),
• Brassica rapa L. (Br),
• Brassica repanda (Willd.) (Be),
• Brassica tournefortii Gouan. (2 accessions, Bt1 and Bt2),
• Carrichtera annua (L.) DC. (Ca),
• Diplotaxis acris Boiss. (Da),
• Diplotaxis erucoides (L.) DC. (De),
• Diplotaxis harra Boiss. (Dh),
• Diplotaxis muralis (L.) DC. (Dm),
• Diplotaxis tenuifolia (L.) DC. (Dt),
• Diplotaxis tenuisiliqua Delile (Ds),
• Diplotaxis viminea (L.) DC. (Dv),
• Eruca sativa Mill. (Es),
• Hirschfeldia incana (L.) Lagr.-Foss (2 accessions HIR1 and HIR3),
• Moricandia arvensis (L.) DC. (Ma),
• Moricandia moricandioides (Boiss.) Heywood (Mm),
• Moricandia nitens E.Durand & Barratte (Mn),
• Moricandia sinaica Boiss. (Msi),
• Moricandia spinosa Pomel (Mp),
• Moricandia suffruticosa (Desf.) Coss. & Durieu(Ms),
• Raphanus raphanistrum L. (Rr),
• Raphanus sativus subsp sativus (L.) (Rs)
• Sinapis alba L. (Sa)
• Gynandropsis gynandra (L.) Briq. /Cleome gynanadra L.
  

Analysis/measurements:

• gas exchange by Li6800 IRGA measuring A-ci response and calculation of CO₂ compensation point
• primary metabolite pattern by GCMS
• element analysis (CN ratio, ¹³C) by EA-IRMS
• vein density (vein length per area)
• PEPC activity in leaf extracts by spectrophotometry
• Specific leaf area (area per dry weight)
• analysis of leaf cross sections by light microscopy and determination of organelle area in bundle sheath facing vein or mesopyll/intercellular space

Data presentation: This ARC contains the data measured per single plant and the raw data for the GCMS and EA-IRMS analysis.

Publication:
Results and discussion of the presented data has been published in the following paper:
Schlüter, U., Bouvier, J. W., Guerreiro, R., Malisic, M., Kontny, C., Westhoff, P., Stich, B. & Weber, A. P. M. (2023), Brassicaceae display diverse photorespiratory carbon recapturing mechanisms, Journal of Experimental Botany, https://doi.org/10.1093/jxb/erad250
Analysis of the genomes of the selected species/taxa can be found in the following paper:
Guerreiro R, Bonthala VS, Schlüter U, Triesch S, Weber APM, Stich B. 2023. A genomic panel for studying C3–C4 intermediate photosynthesis in the Brassiceae tribe. Plant, Cell & Environment https://doi.org/10.1111/pce.14662.
Analysis of the GLDP1 promoter and the potential involvement of transposible elements in evolution of C₃-C₄ phenotype in the Brassicaceae is discussed in:
Triesch S, Denton AK, Buchmannn JP, Reichel-Deland V, Guerreiro R, Schlüter U, Weber APM. 2023. Transposable elements contribute to the establishment of the glycine shuttle in Brassicaceae species. doi: https://doi.org/10.1101/2022.12.06.519256