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Promoter induction.md 449 B

Promoter induction

To enable induction of the Prha promoter, the rhamnose activator rhaS was constitutively expressed by the J23119 promoter from the replicative plasmid pSHDY (AddGene Plasmid #137661, (Behle et al., 2020)), which was transferred to Synechcoystis via triparental mating (Behle et al., 2020). This plasmid was constructed using the restriction sites of the BioBrick and NeoBrick standards and carries a spectinomycin resistance.