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assays/GC-MSMeasurementsForTheQuantificationOfSqualene/README.md

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 **Figure 2** Squalene concentrations [mg l-1 OD750-1] in response to gene overexpressions. Values are represented as the means of three biological replicates, standard deviations are shown. WT represents the *Synechocystis* sp. PCC 6803 wild type, while the control strain is *Synechocystis* sp. PCC 6803 Δshc pSHDY rhaS, from which the overexpression strains were constructed by inserting an additional copy of the specified gene under the control of the rhamnose-inducible promoter Prha into its genome. Asterisks ( * ) represent the p-value of the two-sided t-test between the respective strain and the control strain at the same rhamnose concentration (* denotes a value of p<0.05, ** denotes p<0.01 and *** denotes p<0.001). Samples were measured after three days of incubation with the specified concentration of rhamnose as an inducer.
 
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+**Figure 5** Timeseries of squalene production in sqs overexpression strain. Squalene production and OD750 of *Synechocystis* Δshc pEERM Prha sqs pSHDY rhaS in a 30 ml flask culture in mg l-1 over a period of two weeks after induction with 5 mM rhamnose to trigger overexpression of the squalene synthase (sqs). Means and standard deviations of three biological replicates are shown.
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assays/MetabolicModeling/README.md

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 **Figure 1** Overview of fluxes predicted to change upon increased squalene production. Blue arrows indicate an increased flux and red arrows a decreased flux, respectively. Black arrows indicate no change. Reactions with no flux have a dotted line. The numbers indicate the maximum fold change of the corresponding flux. It is stated that this is not a minimal network but a part of the genome-scale model and not all active reactions are shown. 13DPG, 1;3-bisphosphoglycerate; 2OG, 2-oxoglutarate; 2PGL, 2-phosphoglycolate; AcCoA, acetyl-CoA; ATP synth., ATP synthase; CDP-ME, 4-(cytidine 5′-diphospho)-2-C-methyl-D-erythritol; CDP-MEP, 2-phospho-4-(cytidine 5′-diphospho)-2-C-methyl-D-erythritol; Cit, citrate; Cytb6f, cytochrome b6f complex; DHAP, dihydroxyacetone phosphate; DMAPP, dimethylallyl diphosphate; DXP, 1-deoxy-D-xylulose 5-phosphate; E4P, erythrose 4-phosphate; F6P, fructose 6-phosphate; Fdox, ferredoxin (oxidized); Fdred, ferredoxin (reduced); FDP, fructose 1;6-biphosphate; FNR, ferredoxin-NADP+ reductase; FPP, farnesyl pyrophosphate; Fum, fumarate; G3P, glyceraldehyde 3-phosphate; Glc, D-glycerate; Glx, glyoxylate; Gly, glycolate; GPP, geranyl pyrophosphate; HMBPP, 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate; IsoCit, isocitrate; IPP, isopentenyl diphosphate; Mal, malonate; MEcPP, 2-C-methyl-D-erythritol 2;4-cyclodiphosphate; MEP, 2-C-methyl-D-erythritol 4-phosphate; NDH, NADPH dehydrogenase; OAA, oxaloacetate; PC, plastocyanin; PEP, phosphoenolpyruvate; PG2, 2-phosphoglycerate; PG3, 3-phosphoglycerate; Pi, orthophosphate; PPi, diphosphate; PQ, plastoquinone; PQH2, plastohydroquinone; PSI, photosystem I; PSII, photosystem II; Pyr, pyruvate; R5P, ribose 5-phosphate; Ru5P, ribulose 5-phosphate; RuBP, ribulose 1;5-biphosphate; S17DP, sedoheptulose 1;7-bisphosphate; S7P, sedoheptulose 7-phosphate; Succ, succinate; X5P, xylulose 5-phosphate.
 
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 https://www.frontiersin.org/files/Articles/1024981/fpls-14-1024981-HTML/image_m/fpls-14-1024981-g001.jpg
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assays/PigmentQuantification/README.md

 
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 **Figure 3** Relative change in chlorophyll (left) and carotenoid (right) concentrations [mg l-1 OD750-1] of the overexpression strains compared to the Δshc control strain. Values are represented as the means of three biological replicates. WT represents the Synechocystis sp. PCC 6803 wild type, while the control strain is Synechocystis sp. PCC 6803 Δshc pSHDY rhaS, from which the overexpression strains were constructed by inserting an additional copy of the specified gene under the control of the rhamnose-inducible promoter Prha into its genome. Asterisks (*) represent the p-value of the two-sided t-test between the respective strain and the control strain at the same rhamnose concentration (* denotes a value of p<0.05, ** denotes p<0.01 and *** denotes p<0.001). Samples were measured after three days of incubation with the specified concentration of rhamnose as an inducer.
 
 
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 https://www.frontiersin.org/files/Articles/1024981/fpls-14-1024981-HTML/image_m/fpls-14-1024981-g003.jpg