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CEPLAS
Germann-2023
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dcd97e5d
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dcd97e5d
authored
8 months ago
by
Dominik Brilhaus
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remove mermaid and data statement
parent
0c3c4004
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Pipeline
#5173
passed
8 months ago
Stage: arc_json
Stage: quality_report_generator
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@@ -13,99 +13,3 @@ Germann AT, Nakielski A, Dietsch M, Petzel T, Moser D, Triesch S, Westhoff P and
## License
Copyright © 2023 Germann, Nakielski, Dietsch, Petzel, Moser, Triesch, Westhoff and Axmann. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
## Data availability
The original contributions presented in the study are included in the article/Supplementary Material. Further inquiries can be directed to the corresponding author.
## ARC structure
```
mermaid
%%{
init: {
'theme': 'base',
'themeVariables': {
'background': '#fff',
'lineColor': '#2d3e50',
'primaryTextColor': '#2d3e50'
}
}
}%%
flowchart TD
subgraph Legend
l1(Sample)
l2>Process]
l3(Material)
l4(Data)
end
subgraph Studies
subgraph study\*Synechocystis*
s1(*Synechocystis* mutants)---pr1>growth]-->cl1(mutant cultures)
end
subgraph study\Englund-2014
nz1(Pia Lindberg)---pr9>gift]-->nz2(plasmid pEERM4 Cm)
end
subgraph study\PlasmidAndStrainConstruction
nz2---pr10>cloning of genes]-->s2(Plasmid Constructs)
nz3(replicative plasmid pSHDY)---pr11>cloning of rhamnoze activator]-->s3(plasmid for promoter induction)
s2---pr12>transformation of *Synechocystis*]
s3---pr12-->s1
end
subgraph study\KnoopAndSteuer-2015
nz4(Ralf Steuer)---pr15>send]-->nz5(extended version of a genome-scale stoichiometric network model of *Synechocystis*)
end
end
subgraph Assays
subgraph assay\AbsorptionSpectraAndODMeasurements
cl1---pr2>measurement of Absorption Spectra and OD]-->d1(Absorption Spectra and OD values)
end
subgraph assay\DCWMeasurements
cl1---pr3>measurement of DCW]-->d2(DCW values)
end
subgraph assay\PigmentQuantification
cl1---pr5>sampling and measurement of absorbance maximum]-->a2(Pigment Quantification)
end
d1-->|normalization|a2
d2-->|normalization|a2
subgraph assay\GC-MSMeasurementsForTheQuantificationOfSqualene
cl1---pr4>GC-MS measurement]-->a1(Quantification of Squalene)
end
subgraph assay\qPCR
cl1---pr6>RNA extraction]-->a3(RNA)---pr7>cDNA synthesis]-->a4(cDNA)---pr8>qRT-PCR]-->a5(qRT-PCR results)
end
subgraph assay\cPCR
s1---pr13>cPCR]-->a6(cPCR results)---pr14>gel electrophoresis]-->a7(gel results)
end
subgraph assay\MetabolicModeling
nz5---pr16>metabolic modeling and simulations]-->a8(amplification targets)---pr10
end
%% Defining node styles
classDef S fill:#b4ce82, stroke:#333;
classDef M fill:#ffc000;
classDef D fill:#c21f3a,color:white;
classDef P stroke-width:0px;
%% Assigning styles to nodes
class s1,l1,cl1 S;
class l3,nz2,s2,nz3,s3,a3,a4 M;
class l4,nz5,d1,d2,a1,a2,a5,a6,a7,a8 D;
class l2,pr1,pr2,pr3,pr4,pr5,pr6,pr7,pr8,pr9,pr10,pr11,pr12,pr13,pr14,pr15,pr16 P;
%% Box style
style Studies fill:#fff, stroke-width:2px, stroke:#333;
style Assays fill:#fff, stroke-width:2px, stroke:#333;
end
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