Figure 2 caption
Figure 2 Squalene concentrations [mg l-1 OD750-1] in response to gene overexpressions. Values are represented as the means of three biological replicates, standard deviations are shown. WT represents the Synechocystis sp. PCC 6803 wild type, while the control strain is Synechocystis sp. PCC 6803 Δshc pSHDY rhaS, from which the overexpression strains were constructed by inserting an additional copy of the specified gene under the control of the rhamnose-inducible promoter Prha into its genome. Asterisks ( * ) represent the p-value of the two-sided t-test between the respective strain and the control strain at the same rhamnose concentration (* denotes a value of p<0.05, ** denotes p<0.01 and *** denotes p<0.001). Samples were measured after three days of incubation with the specified concentration of rhamnose as an inducer.
Figure 2 source
Figure 5 caption
Figure 5 Timeseries of squalene production in sqs overexpression strain. Squalene production and OD750 of Synechocystis Δshc pEERM Prha sqs pSHDY rhaS in a 30 ml flask culture in mg l-1 over a period of two weeks after induction with 5 mM rhamnose to trigger overexpression of the squalene synthase (sqs). Means and standard deviations of three biological replicates are shown.
Figure 5 source
### Supplementary Figure 2 caption
Supplementary Figure 2. GC-MS calibration curve for squalene after extraction of 50, 25, 12.5, 6.25 and 3.125 µM of squalene using the method for squalene extraction from Synechocystis cells. Relative response is in relation to the 25 µM β-sitosterol standard, which was solved in the acetone used for extraction.
Supplementary Figure 2 source
https://www.frontiersin.org/articles/10.3389/fpls.2023.1024981/full#supplementary-material