ISA table for PlantGrowthConditions - Sterilization

studies/Plant Growth Conditions/protocols/PlantGrowthConditions.md

+## Plant Growth Conditions
+
+
+A. thaliana Col-0 wild-type (N60000) was obtained from the Nottingham Arabidopsis Stock Centre (NASC). *Arabidopsis thaliana* Col-0 seeds were sterilized using 70% ethanol and bleach. Seeds were submerged in 70% ethanol and left shaking at 40 rpm for 14 minutes. Ethanol was removed before the seeds were submerged in 8.3% sodium hypochlorite (Roth) containing 1 μl of Tween 20 (Sigma-Aldrich) and left shaking at 40 rpm for 4 minutes. Under sterile conditions, the seeds were washed 7x times and finally taken up with sterile 10 mM MgC12. Seeds were left for stratification at 4 °C for 3 days. Seed sterility was confirmed by plating approx. 100 seeds on a 50% TSA plate.
\ No newline at end of file