Plant growth conditions

studies/Plant Growth Conditions/protocols/Agar-media.md

+## Agar-media
+
+Surface-sterilized and stratified *A. thaliana* Col-0 seeds were sown on plates containing
+1% agar (Bacto Agar, Difco) in 1⁄2 MS medium supplemented with 0.5% sucrose and placed
+vertically in a climate chamber (Panasonic, MLR-352) and grown for six days (10 h light, 21 °C; 14 h dark, 19 °C). Using a forceps, uniform seedlings were then transferred to freshly prepared 1⁄2 MS plates without sucrose.
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studies/Plant Growth Conditions/protocols/Flowpot.md

+## Flowpot
+
+Flowpots were assembled and inoculated as described below. Each Flowpot was first flushed with 50 ml sterile MiliQ water and then 50 ml half strength Murashige and Skoog medium with vitamins (1⁄2 MS; 2.2 g/l, Duchefa Biochemie, 0.5 g/l MES, pH 5.7) containing the bacterial inoculum. Per Flowpot, five surface-sterilized and stratified *A. thaliana* Col-0 seeds were pipetted. Microboxes were then incubated in a light cabinet under short day conditions (10 h light at 21 °C, 14 h dark at 19 °C) for 14 days and randomized every 2–3 days.
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