README.md

Figure 2. Diversity and region-specificity of metabolites along the root axis

(A) Principal-component analysis (PCA) of metabolites extracted from the full-length root, and segments 0–2, 2–4, and 4–6 cm from the root tip. The colors of clusters correspond to regions of the root depicted in the plant cartoon in Figure 1A. Centroids are indicated for each cluster of root region. (B) Heatmap depicting the enrichment or depletion of indicated metabolites in respective root segments. Clustering performed based on average linkage Pearson hierarchical clustering of metabolites. Clusters are indicated in red roman numbers. Colors on the scale bar indicate the level of enrichment or depletion of each metabolite across the root segments.

Figure 4. Metabolite profiles of root segments from sweet2, sweet4, and swee11;12 are distinct from WT

(A) Principal-component analysis (PCA) of total metabolites extracted from the whole root and root segments 2, 4, and 6 cm above the root tip of sweet2, sweet4, sweet11;12, and WT plants. WT: N = 16, sweet2: N = 19, sweet4: N = 20, sweet11;12: N = 21. (B) Relative abundance of metabolite superclasses significantly (p value < 0.05) enriched and depleted in each root segment of sweet mutants compared with corresponding segments of WT. (C) Heatmap depicting the enrichment (or depletion) of indicated metabolites in respective root segments for WT and sweet plants (abbreviated as swt). Heatmap generated based on average linkage Pearson hierarchical clustering of metabolites and root segments. Clusters are indicated in red roman numbers. Colors indicate the level of enrichment or depletion of each metabolite across the plant genotypes and root segments.