README.md



Table 1. SWEETs accumulation along the longitudinal region of roots from A. thaliana grown on ½ Murashige-Skoog (½ MS) media supplemented with or without sucrose, and ArtSoil


SWEET
½ MS − sucrose
½ MS + sucrose
ArtSoil


1
regions below 2 cm
regions below 2 cm
regions below 2 cm


2
regions below 0.05 cm and above 2 cm
regions below 0.05 cm and above 2 cm
entire root


3
not tested
not tested
not tested


4
not detected
regions above 2 cm (patchy)
not detected


5
not tested
not tested
not tested


6
not tested
not tested
not tested


7
not detected
not detected
not detected


8
not detected
not detected
not detected


9
not tested
not tested
not tested


10
not tested
not tested
not tested


11
regions below 2 cm
at 2.5–3 cm region
regions below 2 cm


12
regions below 2 cm
regions 2 cm and above
regions 2 cm and above


13
regions below 2.5 cm
regions below 2.5 cm
regions below 2.5 cm


14
not tested
not tested
not tested


15
not tested
not tested
not tested


16
not tested
not tested
not tested


17
entire root
entire root
entire root


Key Resources Table


REAGENT or RESOURCE
SOURCE
IDENTIFIER


Oligonucleotides


AtSWEET4-XbaI-F
IDT
GCAGGTCGACTCTAGAAGTGGTTCCACG GAGATGACG


AtSWEET4-BamHI-R
IDT
CGGTACCCGGGGATCCAGCTGAAACTCG TTTAGCTTGTCC


AtSWEET5-XbaI-F
IDT
GCAGGTCGACTCTAGATTAGGACTGACAC CAGCGATGC


AtSWEET5-BamHI-R
IDT
CGGTACCCGGGGATCCAGCCTGGCCAAG TTCGATTC


AtSWEET7-XbaI-F
IDT
GCAGGTCGACTCTAGAATTCAGGCTTGGC GTAACTTG


AtSWEET7-BamHI-R
IDT
CGGTACCCGGGGATCCAACATTGTTAGGT TCTTGGTTGG


AtSWEET8-XbaI-F
IDT
GCAGGTCGACTCTAGAACCATGACAATTT GGCTCCGAG


AtSWEET8-BamHI-R
IDT
CGGTACCCGGGGATCCAACCCTCTCCGT AGCAGAAATC


Recombinant DNA


pMDC163 vector
Arabidopsis Biological Resource Center https://abrc.osu.edu

TAIR accession Vector:1009003758


promoterless GUSplus vector
Yang et al.81
N/A