Ceplas dm viktoria

assigned to @brilator

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flowchart TD
    Loo-2024[Loo-2024]
    Loo-2024-->STUDY_ArtSoilPreparation
    subgraph STUDY_ArtSoilPreparation[Study:ArtSoilPreparation]
        ArtSoil-Preparation[ArtSoil-Preparation]
        PlantGrowthConditions[PlantGrowthConditions]
    end
    Loo-2024-->STUDY_PlantMaterialAndGrowthConditions
    subgraph STUDY_PlantMaterialAndGrowthConditions[Study:PlantMaterialAndGrowthConditions]
        Sterilization[Sterilization]
        WashingAndStratification[WashingAndStratification]
        GrowthConditions[GrowthConditions]
    end
    Loo-2024-->STUDY_CD-Rhizotron
    subgraph STUDY_CD-Rhizotron[Study:CD-Rhizotron]
        SynComPreparation[SynComPreparation]
        CD-Rhizotron[CD-Rhizotron]
    end
    subgraph ASSAY_AmpliconSequencingDataAnalysis[Assay:AmpliconSequencingDataAnalysis]
        DataAnalysis[DataAnalysis]
    end
    subgraph ASSAY_ANOVAForDifferentialEnrichmentOfMetabolites[Assay:ANOVAForDifferentialEnrichmentOfMetabolites]
        ANOVA[ANOVA]
    end
    subgraph ASSAY_GUSHistochemicalStaining[Assay:GUSHistochemicalStaining]
        GUS-Staining-Solution[GUS-Staining-Solution]
        PlantGrowth[PlantGrowth]
        Staining[Staining]
    end
    subgraph ASSAY_ConstructionOfGUSReporterLines[Assay:ConstructionOfGUSReporterLines]
        GeneAmplification[GeneAmplification]
        Cloning[Cloning]
        FloralDipping[FloralDipping]
        Selection[Selection]
    end
    subgraph ASSAY_QuantifyMetabolicActivitiesUsingPAS[Assay:QuantifyMetabolicActivitiesUsingPAS]
        PAS[PAS]
    end
    subgraph ASSAY_MicrobiotaProfiling[Assay:MicrobiotaProfiling]
        SoilRootSeparation[SoilRootSeparation]
        Centrifugation[Centrifugation]
    end
    subgraph ASSAY_RootMetaboliteProfiling[Assay:RootMetaboliteProfiling]
        GC-MS[GC-MS]
    end
    subgraph ASSAY_DNAExtractionAndLibraryPreparation[Assay:DNAExtractionAndLibraryPreparation]
        DNAExtraction[DNAExtraction]
        PCRreaction1[PCRreaction1]
        PCR1[PCR1]
        EnzymeDigestion[EnzymeDigestion]
        PCRreaction2[PCRreaction2]
        PCR2[PCR2]
        GelElectrophoresis[GelElectrophoresis]
        Sequencing[Sequencing]
    end
    subgraph ASSAY_FluxBalanceAnalysisToDefinePathways[Assay:FluxBalanceAnalysisToDefinePathways]
        FBA[FBA]
    end
    subgraph ASSAY_CorrelationBetweenMicrobesAndMetabolites[Assay:CorrelationBetweenMicrobesAndMetabolites]
    end
    ArtSoil-Preparation-->|1|PlantGrowthConditions
    Sterilization-->|1|WashingAndStratification
    WashingAndStratification-->|1|PlantGrowthConditions
    WashingAndStratification-->|1|GrowthConditions
    WashingAndStratification-->|1|CD-Rhizotron
    GrowthConditions-->|1|FloralDipping
    GrowthConditions-->|1|GC-MS
    SynComPreparation-->|1|CD-Rhizotron
    CD-Rhizotron-->|1|SoilRootSeparation
    PlantGrowth-->|10|Staining
    PlantGrowth-->|3|GC-MS
    GeneAmplification-->|6|Cloning
    Cloning-->|7|FloralDipping
    FloralDipping-->|7|Selection
    Selection-->|7|PlantGrowth
    SoilRootSeparation-->|1|Centrifugation
    Centrifugation-->|1|DNAExtraction
    DNAExtraction-->|1|PCRreaction1
    PCRreaction1-->|1|PCR1
    PCR1-->|1|EnzymeDigestion
    EnzymeDigestion-->|1|PCRreaction2
    PCRreaction2-->|1|PCR2
    PCR2-->|1|GelElectrophoresis
    GelElectrophoresis-->|1|Sequencing
    Sequencing-->|1|DataAnalysis
    Sequencing-->|1|PAS

classDef inv fill:#6c7885,color:#2d3e50,font-weight:bold;
classDef study fill:#62d4c1,color:#2d3e50,font-weight:bold;
classDef assay fill:#ffd34d,color:#2d3e50,font-weight:bold;
classDef process fill:#D46275,color:#2d3e50,font-weight:;
class Loo-2024 inv;
class STUDY_ArtSoilPreparation,STUDY_PlantMaterialAndGrowthConditions,STUDY_CD-Rhizotron study;
class ASSAY_AmpliconSequencingDataAnalysis,ASSAY_ANOVAForDifferentialEnrichmentOfMetabolites,ASSAY_GUSHistochemicalStaining,ASSAY_ConstructionOfGUSReporterLines,ASSAY_QuantifyMetabolicActivitiesUsingPAS,ASSAY_MicrobiotaProfiling,ASSAY_RootMetaboliteProfiling,ASSAY_DNAExtractionAndLibraryPreparation,ASSAY_FluxBalanceAnalysisToDefinePathways,ASSAY_CorrelationBetweenMicrobesAndMetabolites assay;
class ArtSoil-Preparation,PlantGrowthConditions,Sterilization,WashingAndStratification,GrowthConditions,SynComPreparation,CD-Rhizotron,DataAnalysis,ANOVA,GUS-Staining-Solution,PlantGrowth,Staining,GeneAmplification,Cloning,FloralDipping,Selection,PAS,SoilRootSeparation,Centrifugation,GC-MS,DNAExtraction,PCRreaction1,PCR1,EnzymeDigestion,PCRreaction2,PCR2,GelElectrophoresis,Sequencing,FBA process;

mentioned in commit e7d69d3a

merged