add bacterial and plant material study and protocol

studies/BacterialAndPlantMaterial/protocols/BacterialAndPlantMaterialProtocol.md

+## Bacterial and plant material
+
+Bacterial strains were grown in tryptic soy broth (15 g l−1, TSB, Sigma-Aldrich) liquid medium or on agar plates containing 15 g l−1 of Bacto Agar (Difco) at 25 °C. *Mesorhizobium* strains LjNodule210, LjNodule215 and LjNodule218, isolated from *Lj* root nodules, were cultured in TY medium (5 g l−1 tryptone, 3 g l−1 yeast extract) supplemented with 10 mM CaCl2 or in YMB medium (5 g l−1 mannitol, 0.5 g l−1 yeast extract, 0.5 g l−1 K2HPO4·3H2O, 0.2 g l−1 MgSO4·7H2O, 0.1 gl l−1 NaCl). The composition of synthetic bacterial communities (SynComs) is listed in Supplementary Table 1. *Lj* ecotype Gifu B-129 was used as wild-type. Symbiosis-deficient mutant *nfr5-2* (Madsen, E. B. et al., 2003) and flagellin receptor-deficient mutant *fls2* (LORE1-30003492) (Mun, T. et al., 2016) were derived from the Gifu B-129 genotype. For *At*, ecotype Columbia-0 was used as wild-type. Mutant genotypes *fls2* (Zipfel, C. et al., 2004), *bbc* (Xin, X. F. et al., 2016), *deps* (Tsuda, K. et al., 2009) and *cyp79b2 cyb79b3* (Zhao, Y. et al., 2002) were available in our seed stock. *L. corniculatus* seeds, cultivated in the North-Western German lowland, were retrieved from Rieger-Hofmann GmbH, Blaufelden-Raboldshausen, Germany. *A. lyrata* MN47 seeds were a gift from J. de Meaux, University of Cologne.
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