Using CRISPR-Cas9 to generate lwd1 mutants to confirm LWD1 as the gene underlying the eam7 locus. Mutants were genotyped by Sanger sequencing.
study overview
T0 mutant plants (from study "generating_lwd1_mutants"
-> assay: mutant_genotyping
output: T0 mutant genotype info
-> assay: plant propagation
output: T1 mutant seeds
-> assay: plant_genotyping
output: T1 mutant genotype info
-> assay: plant propagation
output: T2 mutant seeds (used in studies gene_expression_lwd1_mutants" and "phenotyping_lwd1_mutants)