README.md

Test diurnal gene expression of flowering time genes in Bowman and Bowman-derived introgression lines over development.

Genotypes BW, BW(Ppd-H1), BW(eam7), and BW(Ppd-H1,eam7) were grown under SD conditions. The youngest, fully elongated leaf was sampled at 19, 26, 33, 40, 47, and 59 DAE.

Gene expression of FT1, FT2, FT3 and VRN1 were tested.

study overview

input: BW, BW(Ppd-H1), BW(eam7), BW(Ppd-H1,eam7) grown under SD

-> assay: leaf_sampling
	output: frozen leaf tissue (3 biol. replicates)
	
	-> assay: RNA_extraction
		output: RNA samples 
		
		-> assay: cDNA_synthesis
			output: cDNA samples
			
			-> assay: qRT-PCR
				output: gene expression data (2 techn. replicates)

Results can be found in Supplemental Figure S3A-D.