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Gesa Helmsorig authoredGesa Helmsorig authored
README.md 1.01 KiB
Biparental mapping to identify eam7
The F2 generation of a cross between BW(eam7) and BW(Ppd-H1) was used to identify and reduced the introgression area of eam7 and to reduce the number of candidate genes to a minimum. CAPS marker were designed based on the 50k Illumina Infinium iSelect SNP Array (Bayer et al. 2017).
study overview
flowchart TD;
A[FT2 crosses and parent plants] --> |plant_phenotyping | B[flowering time of F2 plants and parents]
A --> |DNA_extraction| C[DNA samples for F2 plants and parents]
C --> | biparental_mapping | D[- CAPS marker results
- reduced eam7 introgression area
- target genes for eam7]
B --> D
Publication:
Results can be found in Figure 4, and Supplemental Tables S2, S3, S5.
References
Bayer MM, Rapazote-Flores P, Ganal M, Hedley PE, Macaulay M, Plieske J, Ramsay L, Russell J, Shaw PD, Thomas W, et al. Development and evaluation of a barley 50k iSelect SNP array. Front Plant Sci. 2017:8:1792. https://doi.org/10.3389/fpls.2017.01792