restructured assay

assays/biparental_mapping/dataset/eam7_introgression_area.md

+## reduced *eam7* introgression:
+
+
+| marker start | marker end | Mbp start | Mbp end | size (Mbp) |
+| - | - | - | - | - |
+| JHI-Hv50k-2016-390119 | JHI-Hv50k-2016-401705 | 96.64 | 382.8 | 286.16 |
\ No newline at end of file

assays/biparental_mapping/protocols/CAPS_marker_analysis.md

@@ -28,3 +28,7 @@
 | ------ | ------ | ------ | ------ | ------ | ------ | ------ |
 | M-PPD-H1 | F | ACGTGAATGGTGGATCGGC | BstUI | 60°C | 506 |  433, 73 | 
 | | R | TATAGCTAGGTGCGTGGCG | 						
+
+**Analysis:**
+
+- based on observed recombinations in combination with flowering time data, the eam7 introgression area can be redefined
\ No newline at end of file

assays/biparental_mapping/protocols/target_gene_identification.md

 ## *eam7* target gene identification
 
-- based on the CAPS marker results, the introgression area could be reduced to 2.98 cM/286 Mbp (Morex V3, Mascher et al. 2021)
-
-Flanking markers: 
-
-| marker | position in MorexV3 (bp) |
-| - | - |
-| JHI-Hv50k-2016-390119 | 96639872 |
-| JHI-Hv50k-2016-401705 | 382834297 |
-
-
-- this region contains 1084 high confidence (HC) genes
+- the reduced introgression area contains 1084 high confidence (HC) genes
 - Arabidopsis (Araport11, Cheng et al. 2017) homologs were indentified via BLASTP
 - 10 of the genes genes were homologous to Arabidopsis genes with functions in the circadian clock -> target_genes
 - 4 of these genes were associated with early flowering in barley or Arabidopsis -> target genes