**dissection of main shoot apices (MSAs) to score the reproductive development of plants**
**Dissection of main shoot apices (MSAs) to score the reproductive development of plants**
Scoring of the development according to the Waddington Stage (Waddington et al., 1983).
Scoring of the development according to the Waddington Stage (Waddington et al., 1983).
**study overview**
**Study overview:**
input: BW, BW(Ppd-H1), BW(eam7), BW(Ppd-H1,eam7) grown under SD and LD
input: BW, BW(Ppd-H1), BW(eam7), BW(Ppd-H1,eam7) grown under SD and LD
-> assay: MSA_dissection
-> assay: MSA_dissection
output: - Waddington Stage by DAE
output: - Waddington Stage by DAE
- spikelet meristem number by W-Stage
- spikelet meristem number by W-Stage
- floret number by W-Stage
- floret number by W-Stage
Results can be found in Figure 1C-E, Supplemental Figure S1 and Supplemental Table S1.
Results can be found in Figure 1C-E, Supplemental Figure S1 and Supplemental Table S1.
**References**
**References:**
Waddington SR, Cartwright PM, Wall PC. A quantitative scale of spike initial and pistil development in barley and wheat. Ann Bot. 1983:51(1):119–130. https://doi.org/10.1093/oxfordjournals.aob.a086434
Waddington SR, Cartwright PM, Wall PC. A quantitative scale of spike initial and pistil development in barley and wheat. Ann Bot. 1983:51(1):119–130. https://doi.org/10.1093/oxfordjournals.aob.a086434