added metadata

assays/mutant_genotyping/protocols/plant_genotyping.md

+**lwd1 T0 and T1 plants **
+
+- DNA was extracted from leaves of all mutants using QIAGEN_DNeasy-Plant_mini protocol
+- plants were tested for possible mutations in the CDS of LWD1 by amplifying the full genomic sequence using the primers LWD1_71us_F (5'-ATCAGACCCCTCCAACGACT-3') and LWD1_21ds_R (5'-TTCCTCTTTCTACCCCACCA-3')
+- the PCR fragments were Sanger sequenced using different primers:
+	- plants transformed with pMGE599/7-97 (approach 1): LWD1_71us_F 
+	- plants transformed with pMGE599/182-1222 (approach 2): LWD1_qPCR114F (5'-CAGCTCATAGCCCATGACAA-3')
+- mutations and the zygosity of the mutations were determined relative to the start codon of LWD1
+
+**only in T1 plants**:
+- PCR was performed on transformation vector to check presence/absence of Cas9 using the primers pMGE599_M13F (5'-GTTTTCCCAGTCACGAC-3') and pMGE599_R (5'-TGTCGACATCGATAAGCTTGAA-3') 
\ No newline at end of file

assays/mutant_genotyping/protocols/plant_genotyping.txt

-- DNA extracted from plants using QIAGEN protocol
-- PCR done with primers lwd f/r
-- sent for Sanger sequencing
-- Sequences were evaluated: mutations in CDS of lwd1?
\ No newline at end of file