Biparental mapping to identify eam7

The F2 generation of a cross between BW(eam7) and BW(Ppd-H1) was used to identify and reduced the introgression area of eam7 and to reduce the number of candidate genes to a minimum. CAPS marker were designed based on the 50k Illumina Infinium iSelect SNP Array (Bayer et al. 2017).

study overview

   flowchart TD;
	A[FT2 crosses and parent plants] --> |plant_phenotyping | B[flowering time of F2 plants and parents]
	A --> |DNA_extraction| C[DNA samples for F2 plants and parents]
	C --> | biparental_mapping | D[- CAPS marker results
	- reduced eam7 introgression area
	- target genes for eam7]
	B --> D

Publication:

Results can be found in Figure 4, and Supplemental Tables S2, S3, S5.

References

Bayer MM, Rapazote-Flores P, Ganal M, Hedley PE, Macaulay M, Plieske J, Ramsay L, Russell J, Shaw PD, Thomas W, et al. Development and evaluation of a barley 50k iSelect SNP array. Front Plant Sci. 2017:8:1792. https://doi.org/10.3389/fpls.2017.01792