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Genome sequencing of blackcurrant (Ribes nigrum)
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MAdLand Project - Erika Csicsely, Oguz Top, Wolfgang Frank et al.
This ARC accompanies the publication in Plant Journal: https://doi.org/10.1111/tpj.17236
DICER-LIKE (DCL) proteins play a central role in plant small RNA (sRNA) biogenesis. The genome of the early land plant Marchantia polymorpha encodes four DCL proteins: MpDCL1a, MpDCL1b, MpDCL3, and MpDCL4. While MpDCL1a, MpDCL3 and MpDCL4 show high similarities to their orthologs in Physcomitrium patens and Arabidopsis thaliana, MpDCL1b shares only a limited homology with PpDCL1b, but it is very similar, in terms of functional domains, to orthologs in other moss and fern species. We generated Mpdclge mutant lines for all MpDCL genes with the CRISPR/Cas9 system and conducted phenotypic analyses under control, salt stress, and phytohormone treatments to uncover specific MpDCL functions. The mutants displayed severe developmental aberrations, altered responses to salt and phytohormones, and disturbed sexual organ development. By combining mRNA and sRNA analyses, we demonstrate that MpDCLs and their associated sRNAs play pivotal roles in regulating development, abiotic stress tolerance and phytohormone response in M. polymorpha. We identified MpDCL1a in microRNA biogenesis, MpDCL4 in trans-acting small interfering RNA generation, and MpDCL3 in the regulation of pathogen-related genes. Notably, salt sensitivity in M. polymorpha is dependent on MpDCL1b and Mpdcl1bge mutants display enhanced tolerance and reduced miRNA expression in response to salt stress. We propose that M. polymorpha employs specific mechanisms for regulating MpDCL1b associated miRNAs under high salinity conditions, potentially shared with other species harboring MpDCL1b homologs.
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Usadellab / Gluconobacter_oxydans_GoxR_Characterization
Creative Commons Attribution 4.0 InternationalCharacterization of the FNR-type regulator GoxR
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CEPLAS / VonDahlen-2023
Creative Commons Attribution 4.0 InternationalGlobal expression patterns of R-genes in tomato and potato
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CEPLAS / DeJager-2024
Creative Commons Attribution 4.0 InternationalTraits linked to natural variation of sulfur content in Arabidopsis thaliana
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CEPLAS / Kindel-2024
Creative Commons Attribution 4.0 InternationalPredmoter — cross-species prediction of plant promoter and enhancer regions
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CEPLAS / Gao-2024
Creative Commons Attribution Non Commercial No Derivatives 4.0 InternationalExploring natural genetic variation in photosynthesis-related traits of barley in the field
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A prototypic ARC that implements all specification standards accordingly
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CEPLAS / Germann-2023
Creative Commons Attribution 4.0 InternationalA systematic overexpression approach reveals native targets to increase squalene production in Synechocystis sp. PCC 6803
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CEPLAS / Loo-2024
Creative Commons Attribution Non Commercial No Derivatives 4.0 InternationalSugar transporters spatially organize microbiota colonization along the longitudinal root axis of Arabidopsis
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CEPLAS / Wanke-2023
Creative Commons Attribution Non Commercial No Derivatives 4.0 InternationalA GH81-type β-glucan-binding protein enhances colonization by mutualistic fungi in barley
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HHU Plant Biochemistry / Samuilov-2018-BOU-PSP
Creative Commons Attribution 4.0 InternationalUpdated -
HHU Plant Biochemistry / Brilhaus-2016-Talinum
Creative Commons Attribution 4.0 InternationalUpdated -
CEPLAS / Chandrasekar-2022
Creative Commons Attribution 4.0 InternationalFungi hijack a ubiquitous plant apoplastic endoglucanase to release a ROS scavenging β-glucan decasaccharide to subvert immune responses
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CEPLAS / FokoKuate-2023
Creative Commons Attribution 4.0 InternationalKinetic data for modeling the dynamics of the enzymes involved in animal fatty acid synthesis
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CEPLAS / Helmsorig-2024-eam7
Creative Commons Attribution Non Commercial No Derivatives 4.0 Internationalearly maturity 7 controls photoperiodic flowering in barley by modifying the diurnal expression pattern of the major photoperiod response gene Ppd-H1.
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HHU_MolPhys_Frommer / Schladt et al. 2024 - NUPs
Creative Commons Attribution 4.0 InternationalIdentification of nuclear pore proteins at plasmodesmata
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