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Commit 19cae735 authored by Dominik Brilhaus's avatar Dominik Brilhaus
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fill RNAseq metadata

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Mature leaves were harvested and immediately frozen in liquid nitrogen. RNA was isolated from ground tissue using the Qiagen RNeasy plant mini kit. Residues of DNA were removed with DNase (New England Biolabs). RNA integrity, sequencing library and fragment size were analyzed on a 2100 Bioanalyzer (Agilent). Libraries were prepared using the TruSeq RNA Sample Prep Kit v2 (Illumina), and quantified with a Qubit 2.0 (Invitrogen).
# Preparation and Sequencing of Illumina Libraries
Libraries were prepared using the TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego,CA, United States) and quantified with a Qubit 2.0 (Invitrogen, Waltham, MA, United States). Samples were multiplexed with 12 libraries per lane and sequenced in paired-end mode (Rapid Run, 150 bp read length) on an Illumina HiSeq 3000 platform, yielding on average ∼26 million reads per library.
Libraries were prepared using the TruSeq RNA Sample Prep Kit v2 (Illumina). Samples were multiplexed with 12 libraries per lane and sequenced in paired-end mode (150 bp read length) on an Illumina HiSeq 3000 platform.
# RNA Extraction
Whole rosettes were harvested and immediately frozen in liquid nitrogen. RNA was isolated from ground tissue using the RNeasyPlant Mini Kit (QIAGEN, Hilden, Germany). Residues of DNAwere removed with DNase (New England Biolabs, Ipswitch, MA,United States). RNA integrity, sequencing library, and fragment size were analyzed on a 2100 Bioanalyzer (Agilent Technologies,Santa Clara, CA, United States).
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