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Commit be1d6eba authored by Dominik Brilhaus's avatar Dominik Brilhaus
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change kallisto index to version used then

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1 merge request!2Re build rnaseq
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INFO /Users/dominikbrilhaus/opt/anaconda3/bin/cwltool 3.1.20240112164112
INFO Resolved 'run.cwl' to 'file:///Users/dominikbrilhaus/gitlab_dataplant/hhu-plant-biochemistry/2018_SamuilovBrilhaus_BOU_DB/runs/kallisto_index/run.cwl'
INFO [job run.cwl] /private/tmp/docker_tmpzx_cvyjo$ docker \
run \
-i \
--mount=type=bind,source=/private/tmp/docker_tmpzx_cvyjo,target=/eVdUYd \
--mount=type=bind,source=/private/tmp/docker_tmp4j2zkjbk,target=/tmp \
--mount=type=bind,source=/Users/dominikbrilhaus/gitlab_dataplant/hhu-plant-biochemistry/2018_SamuilovBrilhaus_BOU_DB/workflows/kallisto_index/kallisto_index.sh,target=/var/lib/cwl/stg5f4efaa0-af92-4686-b8ef-77057f169cc5/kallisto_index.sh,readonly \
--mount=type=bind,source=/Users/dominikbrilhaus/gitlab_dataplant/hhu-plant-biochemistry/2018_SamuilovBrilhaus_BOU_DB/studies/arabidopsis-reference/resources/Arabidopsis_thaliana.TAIR10.28.cdna.all.fa,target=/var/lib/cwl/stg7d833517-acee-4e7a-b2b2-7ed5660b407c/Arabidopsis_thaliana.TAIR10.28.cdna.all.fa,readonly \
--workdir=/eVdUYd \
--read-only=true \
--net=none \
--user=502:20 \
--rm \
--cidfile=/private/tmp/docker_tmpjfamrngf/20240125141342-375856.cid \
--env=TMPDIR=/tmp \
--env=HOME=/eVdUYd \
quay.io/biocontainers/kallisto:0.43.0--hdf51.8.17_2 \
bash \
/var/lib/cwl/stg5f4efaa0-af92-4686-b8ef-77057f169cc5/kallisto_index.sh \
/var/lib/cwl/stg7d833517-acee-4e7a-b2b2-7ed5660b407c/Arabidopsis_thaliana.TAIR10.28.cdna.all.fa \
./out
kallisto, version 0.43.0
When using this program in your research, please cite
Bray, N. L., Pimentel, H., Melsted, P. & Pachter, L.
Near-optimal probabilistic RNA-seq quantification,
Nature Biotechnology 34, 525-527(2016), doi:10.1038/nbt.3519
[build] loading fasta file /var/lib/cwl/stg7d833517-acee-4e7a-b2b2-7ed5660b407c/Arabidopsis_thaliana.TAIR10.28.cdna.all.fa
[build] k-mer length: 31
[build] warning: clipped off poly-A tail (longer than 10)
from 24 target sequences
[build] warning: replaced 698 non-ACGUT characters in the input sequence
with pseudorandom nucleotides
[build] counting k-mers ... done.
[build] building target de Bruijn graph ... done
[build] creating equivalence classes ... done
[build] target de Bruijn graph has 290149 contigs and contains 47713570 k-mers
INFO [job run.cwl] Max memory used: 4931MiB
INFO [job run.cwl] completed success
{
"outdir": [
{
"location": "file:///Users/dominikbrilhaus/gitlab_dataplant/hhu-plant-biochemistry/2018_SamuilovBrilhaus_BOU_DB/runs/kallisto_index/out",
"basename": "out",
"class": "Directory",
"listing": [
{
"class": "File",
"location": "file:///Users/dominikbrilhaus/gitlab_dataplant/hhu-plant-biochemistry/2018_SamuilovBrilhaus_BOU_DB/runs/kallisto_index/out/kallisto_index",
"basename": "kallisto_index",
"size": 1029617006,
"checksum": "sha1$4a16e2114b3e4c291d1d6d9c0e989532948a6a43",
"path": "/Users/dominikbrilhaus/gitlab_dataplant/hhu-plant-biochemistry/2018_SamuilovBrilhaus_BOU_DB/runs/kallisto_index/out/kallisto_index"
}
],
"path": "/Users/dominikbrilhaus/gitlab_dataplant/hhu-plant-biochemistry/2018_SamuilovBrilhaus_BOU_DB/runs/kallisto_index/out"
}
]
}INFO Final process status is success
...@@ -4,5 +4,5 @@ sh_script: ...@@ -4,5 +4,5 @@ sh_script:
path: ../../workflows/kallisto_index/kallisto_index.sh path: ../../workflows/kallisto_index/kallisto_index.sh
in_genome_ref: in_genome_ref:
class: File class: File
path: ../../studies/arabidopsis-reference/resources/Araport11_genes.201606.cdna.fasta path: ../../studies/arabidopsis-reference/resources/Arabidopsis_thaliana.TAIR10.28.cdna.all.fa
out_folder: ./out out_folder: ./out
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