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Commit d13fd05c authored by Dominik Brilhaus's avatar Dominik Brilhaus
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![fpls-14-1182105-g005.jpg](dataset\fpls-14-1182105-g005.jpg)
Source: https://www.frontiersin.org/articles/10.3389/fpls.2023.1182105/full#supplementary-material
**Figure 5**
Principal Component Analysis of the Ricinus proteome of the fractions obtained from the sucrose density centrifugation, representing the total proteins (T1 to T4) and the enriched membrane proteins (M1 to M4). In this PCA plot each point represents the identified proteome of one biological experiment.
![fpls-14-1182105-g006.jpg](dataset\fpls-14-1182105-g006.jpg)
**Figure 6**
Distribution profile of compartment-specific marker proteins in the density-gradient fractions containing the total proteins (T1 to T4) and membrane proteins (M1 to M4). (A) peroxisomes; (B) plastid; (C) mitochondria; (D) ER; (E) nucleus; (F) Golgi; (G) vacuole; (H) cytosol. Relative LFQ represents the sum of Label Free Quantification (LFQ) values for all proteins belonging to an organelle relative to its maximal value.
## Supplementary material
Source for supplementary material: https://www.frontiersin.org/articles/10.3389/fpls.2023.1182105/full#supplementary-material
## "Table 3.xlsx"
......@@ -11,7 +24,6 @@ Deconvolution-based assignment of the MS-identified proteins from etiolated cast
SUPPLEMENTARY TABLE 4
List of proteins found in peroxisomes, mitochondria, and plastids isolated from castor bean etiolated endosperm in this study. For functional annotation of proteins and their assignment to biological processes or metabolic pathways, gene ontology analysis was performed by Uniprot.
## "Image 1.jpg"
SUPPLEMENTARY FIGURE 1
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![fpls-14-1182105-g001.jpg](resources/fpls-14-1182105-g001.jpg)
Figure 1
**Figure 1**
Endosperm morphology in seeds and germinating seedling of R. communis. Photographs were taken from the whole plant (upper panel) and two cotyledons embedded in the endosperm (lower panel) from mature seeds (dry), 24h-imbibed seeds (imb) and from 1- to 7-day old dark-grown castor bean seedlings (1-7d). Scale bar = 1 cm.
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# 2.1 Plant growth conditions
Dry seeds of Ricinus communis var. zanzibariensis were surface- sterilized in a solution of 0.1% (w/v) 8-quinolinol in water for ten minutes and soaked in running tap water over night for seed imbibition. The imbibed seeds were placed on moist vermiculite and incubated at 30°C in the dark (Beevers and Breidenbach, 1974).
Dry seeds of *Ricinus communis* var. *zanzibariensis* were surface- sterilized in a solution of 0.1% (w/v) 8-quinolinol in water for ten minutes and soaked in running tap water over night for seed imbibition. The imbibed seeds were placed on moist vermiculite and incubated at 30°C in the dark (Beevers and Breidenbach, 1974).
- Beevers, H., and Breidenbach, R. W. (1974). “Glyoxysomes,” in Methods in enzymology, Vol 16 Biomembranes, Part A. Eds. S. Fleciher and L. packer (New York London: Academic Press), 565–571.
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